{"product_id":"biolegend-127808","title":"Biolegend, 127808, PE anti-mouse TCR Vα2 Antibody, 100μg","description":"\u003cp\u003eThe TCR alpha (α) chain complexes with the TCR beta (β) chain to form the T cell receptor in 95% of T cells, whereas the remaining 5% of T cells express gamma and delta chains (γ\/δ).  TCR Vα2 is a distinct TCR subfamily found in mice having the a, b, and c haplotypes.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Soluble TCR from mouse CTL clone KB5-C20\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: The B20.1 antibody recognizes most members of the Va2 TCR subfamily in mice having the a, b, and c haplotypes.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Pircher H, et al. 1992. Eur. J. Immunol.. 22:399. Gregoire C, et al. 1991. P. Natl. Acad. Sci. USA 88:8077. Kao C, et al. 2005. Int. Immunol.17:1607. PubMed Steptoe RJ, et al. 2007. J. Immunol. 178:2094. PubMed Rao RR, et al. 2012. Immunity. 36:374. PubMed.\u003cbr\u003e\nProduct Citations: Logan K Smith et al. 2018. Immunity. 48(2):299-312 . PubMed Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed Gargaro M, et al. 2022. Immunity. 55:1032. PubMed Alissafi T, et al. 2018. J Clin Invest. 128:3840. PubMed Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed Sinclair LV et al. 2019. Elife. 8 pii: e44210. PubMed Sartorius R, et al. 2015. EMBO Mol Med. 7: 973 - 988. PubMed Macdougall CE et al. 2018. Cell metabolism. 27(3):588-601 . PubMed Kondo M, et al. 2016. J Immunol. 196: 563 - 572. PubMed Ying Zhang et al. 2017. Cancer cell. 32(3):377-391 . PubMed Landon J Edgar et al. 2018. Cell chemical biology. 26(1):131-136 . PubMed Huang J, et al. 2014. J Immunol. 192:1972. PubMed Pizzolla A, et al. 2016. PLoS One. 11: 0160407. PubMed Lebel MÈ, et al. 2020. Nat Commun. 3.051388889. PubMed Bahmani B, et al. 2018. J Clin Invest. 128:4770. PubMed Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed\u003cbr\u003e\nRRID: AB_1134184 (BioLegend Cat. No. 127807) AB_1134183 (BioLegend Cat. No. 127808)\u003cbr\u003e\nDistribution: On a subset of T cells\u003cbr\u003e\nFunction: In combination of V β, Vα recognizes MHC\/peptide complex on the antigen-presenting cells.\u003cbr\u003e\nInteraction: Associate with Vβ and CD3 complex to form TCR complexes expressing on cell surface.\u003cbr\u003e\nLigand\/Receptor: TCR recognizes MHC\/peptide complex\u003cbr\u003e\nCell Type: T cells\u003cbr\u003e\nBiology Area: Adaptive Immunity, Immunology\u003cbr\u003e\nMolecular Family: TCRs\u003cbr\u003e\nAntigen References: 1. Kubo RT, et al. 1989. J. Immunol.. 142:2736. 2. Pircher H, et al. 1992. Eur. J. Immunol. 22:399.\u003cbr\u003e\nGene ID: 21473\u003cbr\u003e\nUniProt: View information about TCR Valpha2 on UniProt.org\u003cbr\u003e\nClone: B20.1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: T cell receptor Vα 2\u003cbr\u003e\nIsotype: Rat IgG2a, λ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862548631721,"sku":"127808","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-127808","provider":"Iright","version":"1.0","type":"link"}