{"product_id":"biolegend-129804","title":"Biolegend, 129804, PE anti-mouse CD196 (CCR6) Antibody, 100μg","description":"\u003cp\u003eCCR6 is a G-protein linked chemokine receptor which binds the chemokine CCL20\/MIP-3a. It is expressed on B lymphocytes and certain subsets of dendritic cells and T cells. CCR6 is reported to be involved in mucosal immune response and lymphocyte migration and homeostasis.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Armenian Hamster\u003cbr\u003e\nImmunogen: N-terminal peptide (aa. 2-38) of mouse CCR6 fused with GST\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nProduct Citations: Doisne J, et al. 2015. J Immunol. 195: 3937 - 3945.. PubMed Wight AE, et al. 2022. Proc Natl Acad Sci U S A. 119:e2200757119. PubMed Liu QZ, et al. 2018. Front Immunol. 1.131944444. PubMed Kobayashi T, et al. 2019. Cell. 176:982. PubMed Browning LM, et al. 2020. Cell Rep. 33:108219. PubMed Chen J, et al. 2017. J Biol Chem. 292:14532. PubMed Shibata K, et al. 2014. J Immunol. 192:2210. PubMed Huang X, et al. 2020. Cell Host Microbe. 29(2):210-221.e6. PubMed Alves C, et al. 2015. PLoS One. 10: 0142972. PubMed Sundling C, et al. 2021. Immunity. 54(5):988-1001.e5. PubMed Linehan JL et al. 2018. Cell. 172(4):784-796 . PubMed Li Z, et al. 2018. Front Immunol. 9:347. PubMed Martínez‐López M et al. 2019. Immunity. 50(2):446-461 . PubMed Wara AK, et al. 2020. Cell Rep. 33:108550. PubMed Chatterjee S et al. 2017. Cell metabolism. 27(1):85-100 . PubMed Timilshina M, et al. 2017. PLoS One. 10.1371\/journal.pone.0168942. PubMed Suan D et al. 2017. Immunity. 47(6):1142-1153 . PubMed Engel I, et al. 2016. Nat Immunol. 10.1038\/ni.3437. PubMed Zeis P, et al. 2020. Immunity. 53:775. PubMed Shin JW, et al. 2020. Cell Death Dis. 0.667361111. PubMed Mony J, et al. 2014. Mult Scler. . PubMed Zhong C, et al. 2020. Immunity. 52(1):83-95.e4.. PubMed Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed Wang X, et al. 2021. Immunity. 54(6):1123-1136.e8. PubMed Goc J, et al. 2021. Cell. 184:5015. PubMed\u003cbr\u003e\nRRID: AB_1279139 (BioLegend Cat. No. 129803) AB_1279137 (BioLegend Cat. No. 129804)\u003cbr\u003e\nStructure: G-protein linked, seven transmembrane, domain-spanning chemokine receptor; 367 amino acids long and about 75% homology with human CCR6\u003cbr\u003e\nDistribution: Expressed on spleen B cells, immature splenic dendritic cells in Peyer's patches, and some T cell subset\u003cbr\u003e\nFunction: Important in regulating mucosal immune response\u003cbr\u003e\nLigand\/Receptor: CCL20\/MIP-3a\u003cbr\u003e\nCell Type: B cells, Dendritic cells, T cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors, GPCR\u003cbr\u003e\nAntigen References: 1. Rossi D, et al. 1997. J. Immunol. 158:1033. 2. Lukacs N, et al. 2001. J. Exp. Med. 194:551. 3. Cook DN, et al. 2000. Immunity 12:495\u003cbr\u003e\nGene ID: 12458\u003cbr\u003e\nUniProt: View information about CD196 on UniProt.org\u003cbr\u003e\nClone: 29-2L17\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: CD196, BN-1, CKR-L3, DRY6, KY411, STRL22\u003cbr\u003e\nIsotype: Armenian Hamster IgG\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003cbr\u003e\nQ: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?\u003cbr\u003e\nA: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860885852329,"sku":"129804","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-129804","provider":"Iright","version":"1.0","type":"link"}