{"product_id":"biolegend-129906","title":"Biolegend, 129906, PE anti-mouse Tim-4 Antibody, 100μg","description":"\u003cp\u003eTIM4 is a transmembrane protein known as T cell immunoglobulin and mucin domain containing protein-4. It is expressed on antigen-presenting cells and not on T cells.TIM4 binds to TIM1 to promote T cell proliferation by enhancing cell division and reducing apoptosis.TIM4 bind to phosphatidylserine through its FG-CC’ binding cleft in the N-terminal IgV domain to facilitate the clearance of apoptotic cells or bodies.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse TIM4-Ig fusion protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Rodriguez-Manzanet R, et al. 2008. J. Immunol. 180:4706\u003cbr\u003e\nProduct Citations: Castoldi Â, et al. 2023. iScience. 107719:26. PubMed De Schepper S et al. 2018. Cell. 175(2):400-415 . PubMed Samarchith P Kurup et al. 2019. Cell host \u0026amp; microbe. 25(4):565-577 . PubMed\u003cbr\u003e\nRRID: AB_1227799 (BioLegend Cat. No. 129905) AB_1227798 (BioLegend Cat. No. 129906)\u003cbr\u003e\nStructure: Transmembrane protein containing immunoglobulin domain and mucin-like domain\u003cbr\u003e\nDistribution: Unlike the other TIMs, TIM4 is only expressed by antigen-presenting cells, but not by T cells.\u003cbr\u003e\nFunction: TIM4 is expressed on antigen-presenting cells. It provides co-stimulation to promote T cell proliferation and survival and has a possible role in clearance of apoptotic cells by binding phosphatidylserine.\u003cbr\u003e\nInteraction: Phosphatiylserine is the ligand of TIM4 and TIM4 is a ligand of TIM1\u003cbr\u003e\nLigand\/Receptor: Phosphatidylserine, TIM1\u003cbr\u003e\nCell Type: Antigen-presenting cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nAntigen References: 1. Kuchroo VK, et al. 2008. Nat. Rev. Immunol. 8:577 2. Miyanishi M et al. 2007. Nature 450:435 3. Rodriguez-Manzanet R et al. 2008. J. Immunol. 180:4706\u003cbr\u003e\nGene ID: 276891\u003cbr\u003e\nUniProt: View information about Tim-4 on UniProt.org\u003cbr\u003e\nClone: F31-5G3\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: T cell immunoglobulin and mucin domain containing protein-4\u003cbr\u003e\nIsotype: Rat IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46863080980649,"sku":"129906","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-129906","provider":"Iright","version":"1.0","type":"link"}