{"product_id":"biolegend-135228","title":"Biolegend, 135228, PE\/Dazzle™ 594 anti-mouse CD279 (PD-1) Antibody, 100μg","description":"\u003cp\u003eCD279, also known as programmed death-1 (PD-1), is a 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily. PD-1 is expressed on activated splenic T and B cells and thymocytes. It is induced on activated myeloid cells as well. PD-1 is involved in lymphocyte clonal selection and peripheral tolerance through binding its ligands, B7-H1 (PD-L1) and B7-DC (PD-L2). It has been reported that PD-1 and PD-L1 interactions are critical to positive selection and play a role in shaping the T cell repertoire. PD-L1 negative costimulation is essential for prolonged survival of intratesticular islet allografts.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: PD-1 cDNA followed by PD-1-Ig fusion protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE\/Dazzle™ 594 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PE\/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue3, in vivo blocking of PD-1 binding to its ligands2,3, and spatial biology (IBEX)5,6.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed Lázár-Molnár E, et al. 2008. Proc. Natl. Acad. Sci. USA 105:2658. (Block) Liang SC, et al. 2003. Eur. J. Immunol. 33:2706. (FC, IHC, Block) Tobias J, et al. 2020. Front Immunol. 11:895 (FC, ELISA) PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Zeng S, et al. 2023. Front Oncol. 13:1171926. PubMed Orvain C, et al. 2022. Arthritis Res Ther. 24:13. PubMed Chaurio RA, et al. 2022. Immunity. 55:115. PubMed Chen X, et al. 2022. J Extracell Vesicles. 11:e12279. PubMed Zhu X, et al. 2017. Arch Oral Biol. 10.1016\/j.archoralbio.2017.03.010. PubMed Moon S, et al. 2021. J Exp Med. 218:. PubMed Taylor D, et al. 2022. Front Immunol. 13:936129. PubMed \u0026amp;Sacute;ledzi\u0026amp;nacute;ska A, et al. 2020. Immunity. 52(1):151-166.e6.. PubMed Velazquez VM, et al. 2017. Mol Ther Methods Clin Dev. 0.277083333. PubMed Wang C, et al. 2018. Sci Transl Med. 10. PubMed Lee A, et al. 2022. Nat Commun. 13:549. PubMed Zhang L, et al. 2021. Mol Ther. 29:744. PubMed\u003cbr\u003e\nRRID: AB_2566005 (BioLegend Cat. No. 135227) AB_2566006 (BioLegend Cat. No. 135228)\u003cbr\u003e\nStructure: A 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily.\u003cbr\u003e\nDistribution: Induced on splenic T and B lymphocytes, thymocytes, and myeloid cells after stimulation.\u003cbr\u003e\nFunction: Involved in lymphocyte clonal selection and peripheral tolerance, prolonged survival of allografts.\u003cbr\u003e\nLigand\/Receptor: B7-H1 (PD-L1) and B7-DC (PD-L2)\u003cbr\u003e\nCell Type: B cells, T cells\u003cbr\u003e\nBiology Area: Cancer Biomarkers, Immunology, Inhibitory Molecules\u003cbr\u003e\nMolecular Family: CD Molecules, Immune Checkpoint Receptors\u003cbr\u003e\nAntigen References: 1. Nishimura H, et al. 2001. Science 291:319 2. Agata Y, et al. 1996. Int. Immunol. 8:765 3. Liang SC, et al. 2003. Eur. J. Immunol. 33:2706 4. Barber DL, et al. 2006. Nature 439:682 5. Keir ME, et al. 2005. J. Immunol. 175:7372 6. Koehn BH. et al. 2008. J Immunol. 181:5313\u003cbr\u003e\nGene ID: 18566\u003cbr\u003e\nUniProt: View information about CD279 on UniProt.org\u003cbr\u003e\nClone: 29F.1A12\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: PD-1, Programmed Death-1, PDCD1\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866548326569,"sku":"135228","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-135228","provider":"Iright","version":"1.0","type":"link"}