{"product_id":"biolegend-136008","title":"Biolegend, 136008, APC anti-mouse CD140b Antibody, 100μg","description":"\u003cp\u003ePlatelet-derived growth factor receptor-β (PDGFR-β), CD140b, is one of two receptors for platelet-derived growth factors (PDGFs) and binds to all isoforms of PDGFs. PDGFRβ is a receptor tyrosine kinase that forms homodimers or heterodimers on the surface upon ligand binding and phosphorylates substrates. PDGFRs consist of either homodimers of α\/α, β\/β, or heterodimers of α\/β. PDGF receptors, α and β, are single glycoproteins with intracellular tyrosine kinase domain. Their ligand, PDGF, is a mitogen for connective tissue and glial cells. CD140b is expressed on embryonic tissues and mesenchymal-derived cells adult mice. PDGF plays a role in wound healing and acts as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes, and neutrophils.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse PDGFR-β-hIgG1 recombinant fusion protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Additional reported (for the relevant formats) applications include: Western blotting and blocking function2. The LEAF™ purified antibody is recommended for functional assays.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Sano H, et al. 2001. Circulation 103:2955. Sano H, et al. 2002. Am. J. Pathol. 161:135. (Block)\u003cbr\u003e\nProduct Citations: Hepler C et al. 2017. eLife. 6 pii: e27669. PubMed Contreras O, et al. 2023. Front Cell Dev Biol. 11:1017660. PubMed Miller K, et al. 2021. Toxicol Sci. 184:286. PubMed Ajay AK, et al. 2022. Cell Rep. 38:110473. PubMed Wang W, et al. 2019. Cell Metab. 30:174. PubMed Khodeer S, et al. 2022. J Cell Sci. 135: . PubMed Crewe C et al. 2018. Cell. 175(3):695-708 . PubMed Kramann R, et al. 2018. JCI Insight. 3. PubMed Sakihama H, et al. 2021. Arterioscler Thromb Vasc Biol. :ATVBAHA120315558. PubMed Hepler C et al. 2018. eLife. 7 pii: e39636. PubMed Alonso-Herranz L, et al. 2020. Elife. 9:00. PubMed Ohmori T, et al. 2015. Sci Rep. 5: 15676. PubMed Sun X, et al. 2020. eLife. 9:e46756.. PubMed Dahlgren MW et al. 2019. Immunity. 50(3):707-722 . PubMed Miki K, et al. 2021. Nat Commun. 12:3596. PubMed Yu Q, et al. 2016. Cell Res. 10.1038\/cr.2016.85. PubMed Kumashiro Y, et al. 2015. Small. 6: 681 - 687. PubMed Hreha TN, et al. 2020. Physiol Rep. 8:e14401. PubMed Krenzlin H, et al. 2019. J Clin Invest. 130:1671. PubMed Wong PP, et al. 2020. Cell. 181(6):1346-1363.e21. PubMed\u003cbr\u003e\nRRID: AB_2043971 (BioLegend Cat. No. 136007) AB_2268091 (BioLegend Cat. No. 136008)\u003cbr\u003e\nStructure: Beta chain of the platelet-derived growth factor receptor, a receptor tyrosine kinase that forms homo- or hetero-dimers on the surface after ligand binding.\u003cbr\u003e\nDistribution: Expressed on embryonic tissues and mesenchymal-derived cells of adult mouse.\u003cbr\u003e\nFunction: Play a role in wound healing and act as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes and neutrophils.\u003cbr\u003e\nLigand\/Receptor: PDGFs\u003cbr\u003e\nCell Type: Embryonic Stem Cells, Mesenchymal Stem Cells\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors\u003cbr\u003e\nAntigen References: 1. Soriano P, et al. 1994. Genes Dev. 8:1888 2. Takakura N, et al. 1996. J Invest Dermatol. 107:770 3. Yarden Y, et al. 1986. Nature 323:226\u003cbr\u003e\nGene ID: 18596\u003cbr\u003e\nUniProt: View information about CD140b on UniProt.org\u003cbr\u003e\nClone: APB5\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: PDGF receptor-β, PDGFR-β, platelet-derived growth factor receptor beta\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862541160617,"sku":"136008","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-136008","provider":"Iright","version":"1.0","type":"link"}