{"product_id":"biolegend-136204","title":"Biolegend, 136204, PE anti-mouse ESAM Antibody, 200μg","description":"\u003cp\u003eEndothelial cell-selective adhesion molecule (ESAM) is a 55-kD membrane protein composed of two extracellular Ig domains, a single transmembrane domain, and a cytoplasmic domain. ESAM is predominantly expressed at endothelial junctions and on platelets, participating in the migration of neutrophils through the vessel wall by influencing endothelial cell contacts. It impacts vascular permeability and extravasation process. Recently, it was reported that ESAM is a novel marker for murine hematopoietic stem cells (HSCs) in fetal liver. ESAM expression is correlated with HSC activity. The ESAM Hi population was highly enriched for multipotent myeloid-erythroid progenitors and primitive progenitors with lymphpoietic activity, and exclusively reconstituted long-term lymphohematopoiesis in lethally irradiated recipients.\u003cbr\u003e\n200μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse bEND.3 endothelial cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Nasdala I, et al. 2002. J. Bio. Chem. 277:16294\u003cbr\u003e\nProduct Citations: Kang YA, et al. 2023. J Exp Med. 220:. PubMed Wang L, et al. 2021. Cancer Immunol Res. 9:348. PubMed De Simone G, et al. 2021. Immunity. :. PubMed Barbet G, et al. 2018. Immunity. 48:584. PubMed Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed Andreata F, et al. 2021. STAR Protoc. 2:100831. PubMed\u003cbr\u003e\nRRID: AB_1953300 (BioLegend Cat. No. 136203) AB_1953301 (BioLegend Cat. No. 136204)\u003cbr\u003e\nStructure: A 55-kD membrane protein composed of two extracellular Ig domains, a single transmembrane domain, and a cytoplasmic domain.\u003cbr\u003e\nDistribution: Expressed at endothelial junctions, platelets, and murine hematopoietic stem cells.\u003cbr\u003e\nFunction: It impacts vascular permeability and extravasation process. ESAM expression is involved in hematopoietic stem cell (HSC) activity.\u003cbr\u003e\nCell Type: Endothelial cells, Hematopoietic stem and progenitors, Platelets\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: Adhesion Molecules\u003cbr\u003e\nAntigen References: 1. Wegmann F, et al. 2004. Exp Cell Res. 300:121 2. Ooi LAG, et al. 2008. Stem Cells. 27:653 3. Yokota T, et al. 2009. Blood 113(13):2871. Phosphate-buffered solution pH 7.2 containing 0.09% sodium azide.\u003cbr\u003e\nGene ID: 69524\u003cbr\u003e\nUniProt: View information about ESAM on UniProt.org\u003cbr\u003e\nClone: 1G8\/ESAM\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Endothelial cell-selective adhesion molecule\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860860063913,"sku":"136204","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-136204","provider":"Iright","version":"1.0","type":"link"}