{"product_id":"biolegend-136503","title":"Biolegend, 136503, PE anti-mouse CD93 (AA4.1, early B lineage) Antibody, 50μg","description":"\u003cp\u003eCD93 is a 130-140kD C-type lectin like type I transmembrane protein, also known as complement component 1, q subcomponent (C1qR1), C1qRp collectin receptor (C1qRp), or AA4 antigen. It is a receptor expressed on immature B lymphocytes, hematopoietic progenitors and stem cells in adult bone marrow, fetal liver, embryonic yolk sac. CD93 expression level on splenic immature\/transitional B cells is much lower than in bone marrow. It is reinduced during plasma cell differentiation and plays an important role in maintaining plasma cells in bone marrow niches. Immature dendritic cells express CD93 and down-regulate this molecule upon maturation, suggesting a role in uptake of particles by DC. It is also expressed on monocytes, macrophages, and endothelial cells. Macrophages from CD93 (-\/-) mice had a significant phagocytic defect in the clearance of apoptotic cells in vivo , indicating CD93 may contribute to the in vivo clearance of dying cells. Binding of CD93 to C1q remains controversial.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Pre-B lymphoma 70Z\/3\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): McKearn JP, et al. 1984. J. Immunol. 132:332 Hanihara-Tatsuzaawa F, et al. 2014. J Biol Chem. 289:30925. PubMed\u003cbr\u003e\nProduct Citations: Meyer SN, et al. 2020. Immunity. 51(3):535-547. PubMed Moreau J, et al. 2015. Blood. 126: 1184-1192. PubMed Pessoa Rodrigues C, et al. 2021. Sci Adv. 7:. PubMed Yousif AS, et al. 2020. Immunity. 54(2):235-246.e5. PubMed Sarapulov AV, et al. 2020. Front Immunol. 11:599. PubMed Lee JL, et al. 2022. Aging Cell. 21:e13692. PubMed Fredriksson-Lidman K, et al. 2017. PLoS One. 10.1371\/journal.pone.0185509. PubMed Yankova E, et al. 2021. Nature. 593:597. PubMed Hanihara-Tatsuzawa F, et al. 2014. J Biol Chem. 389:30925. PubMed Sabouri Z, et al. 2016. Nat Commun. 7:13381. PubMed Wang J, et al. 2020. Cell. 183(7):1867-1883.e26. PubMed Silva N, et al. 2016. J Immunol. 196: 2591 - 2601. PubMed\u003cbr\u003e\nRRID: AB_1967094 (BioLegend Cat. No. 136503) AB_1967125 (BioLegend Cat. No. 136504)\u003cbr\u003e\nStructure: A 130-140 kD C-type lectin like type I transmembrane protein, also known as complement component 1, q subcomponent (C1qR1), C1qRp collectin receptor (C1qRp), or AA4 antigen.\u003cbr\u003e\nDistribution: Expressed during immature B lymphocytes, hematopoietic progenitors and stem cells in bone marrow, immature dendritic cells, monocytes, macrophages, and endothelial cells.\u003cbr\u003e\nFunction: CD93 plays an important role in maintaining of plasma cells in bone marrow niches. It may play a role in uptake of particles by DC and the in vivo clearance of dying cells by macrophages.\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Endothelial cells, Hematopoietic stem and progenitors, Macrophages, Monocytes\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Steinberger P, et al. 2002. J. Leukoc. Biol. 71:133 2. Chevrier S, et al. 2009. Proc. Nat. Acad. Sci. U. S. A. 106:3895 3. Norsworthy PJ, et al. 2004. J. Immunol. 172:3406 4. Li YS, et al. 1996. Immunity 5:527 5. Szilvassy SJ, et al. 1993. Blood 81:2310\u003cbr\u003e\nGene ID: 17064\u003cbr\u003e\nUniProt: View information about CD93 on UniProt.org\u003cbr\u003e\nClone: AA4.1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: AA4.1, C1qRp, Early B lineage antigen\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862527561897,"sku":"136503","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-136503","provider":"Iright","version":"1.0","type":"link"}