{"product_id":"biolegend-137001","title":"Biolegend, 137001, Purified anti-mouse CD68 Antibody, 50μg","description":"\u003cp\u003eMouse CD68, also known as macrosialin, is an 85-115 kD member of the lysosomal-associated membrane protein (LAMP) family. It is a heavily glycosylated and predominantly intracellular protein, mainly in late endosomes. Macrosialin is the murine homolog to the human macrophage glycoprotein CD68. It is expressed on tissue macrophages, Langerhans cells and at low levels on dendritic cells. Lamp proteins may have functions relating to cell-cell interaction or cell-ligand interaction. The biological function of CD68 is not completely understood.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Purified Con A receptor glycoproteins from the P815 cell line\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: ICFC - Quality testedFC - VerifiedIP, WB, IHC-F - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported (for relevant formats) applications include: immunoprecipitation1, 2, Western Blot1, 2, immunohistochemical staining of frozen sections2 and paraformaldehyde-fixed paraffin-embedded sections3, and spatial biology (IBEX)9,10.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Silva RP, et al. 1999. Biochem. J. 338:687. (IP, WB) Rabinowitz SS, et al. 1991. J. Exp. Med. 174:827. (IP, WB, IHC) Wu J, et al. 2008. P. Natl. Acad. Sci. USA 105:16934. (IHC) Kayama H, et al. 2012. PNAS. 109:5010. PubMed Park S, et al. 2013. Biomaterials. 34:598. PubMed Guiducci C, et al. 2013. J Exp Med. 210:2903. PubMed McKinstry SU, et al. 2014. J Neurosci. 34:9455. PubMed Li X, et al. 2015. J Am Heart Assoc. 6:4. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Guiducci C, et al. 2013. J Exp Med. 210:2903. PubMed Zaman R, et al. 2021. Immunity. :. PubMed Fang W, et al. 2020. Transl Res. 222:28. PubMed Milasan A, et al. 2017. J Am Heart Assoc. 10.1161\/JAHA.117.006892. PubMed Filipello F et al. 2018. Immunity. 48(5):979-991 . PubMed Miho Sato‐Hashimoto et al. 2019. eLife. 8 pii: e42025. PubMed Ruan S, et al. 2023. Front Pharmacol. 14:1088957. PubMed Michurina S, et al. 2023. Int J Mol Sci. 24:. PubMed Ritzel RM, et al. 2023. Sci Adv. 9:eadd1101. PubMed Yin XY, et al. 2023. J Neuroinflammation. 20:69. PubMed Rodriguez Y, et al. 2022. Liver Res. 6:227. PubMed Zhang R, et al. 2023. Stroke Vasc Neurol. . PubMed Dietsch B, et al. 2023. BMC Cancer. 23:436. PubMed Schake MA, et al. 2023. iScience. 26:106760. PubMed Taniguchi M, et al. 2023. Mol Pharmacol. . PubMed Preda MB, et al. 2021. Cell Death Dis. 12:566. PubMed Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed Mathew DJ, et al. 2021. Sci Rep. 11:22880. PubMed Da Mesquita S, et al. 2021. Nature. 593:255. PubMed Meng J, et al. 2022. J Neurosci. 42:4958. PubMed Mugarza E, et al. 2022. Sci Adv. 8:eabm8780. PubMed Block CL, et al. 2022. Cell Rep. 40:111161. PubMed Saraswathula A, et al. 2023. Int Forum Allergy Rhinol. 13:230. PubMed Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed Ohkubo R, et al. 2022. Cell Rep. 41:111803. PubMed Wu J, et al. 2023. Cell Mol Biol Lett. 28:11. PubMed , et al. 2022. JACC Basic Transl Sci. 7:I. PubMed McKinstry S, et al. 2014. J Neurosci. 34:9455. PubMed Spencer WJ, et al. 2019. Proc Natl Acad Sci U S A. 116:13087. PubMed Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed Guimbal S, et al. 2021. Arterioscler Thromb Vasc Biol. 41:e193. PubMed Park S, et al. 2013. Biomaterials. 34:598. PubMed Henjum K, et al. 2020. Sci Rep. 10:10590. PubMed Sherafat A, et al. 2021. Nat Commun. 12:2265. PubMed Gomez-Pastor R, et al. 2017. Nat Commun. 8:14405. PubMed Trus I, et al. 2020. Front Immunol. 2.553472222. PubMed Shen L et al. 2019. Br J Pharmacol. 176(14):2559-2572 . PubMed Tian K, et al. 2021. J Immunol Res. 2021:6297332. PubMed Li X, et al. 2015. J Am Heart Assoc. 6:4. PubMed Brocker CN, et al. 2017. Am J Physiol Gastrointest Liver Physiol. 312:G283. PubMed Di Gioia M, et al. 2020. Nat Immunol. 21:42. PubMed Burrus CJ, et al. 2020. Cell Rep. 30:642. PubMed Lyras EM, et al. 2022. Elife. 11: . PubMed Batool M, et al. 2020. Basic Res Cardiol. 115:68. PubMed Mariot V, et al. 2021. J Cachexia Sarcopenia Muscle. Online ahead of print. PubMed O'Dea KP, et al. 2020. J Extracell Vesicles. 9:1706708. PubMed Varol D et al. 2017. Immunity. 46(6):1030-1044 . PubMed Mori H, et al. 2015. Toxicol Pathol. 43: 883-889. PubMed Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed Silburt J, et al. 2022. J Neuroinflammation. 19:24. PubMed Kim H, et al. 2020. ACS Nano. 14:6519. PubMed Goldblatt DL, et al. 2020. British Journal of Pharmacology. 177(10):2256-2273.. PubMed Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed Tsubaki T, et al. 2018. Oncotarget. 9:11209. PubMed Carvalho K, et al. 2022. J Neuroinflammation. 19:178. PubMed Stafeev I, et al. 2022. Front Nutr. 8:809732. PubMed Shi W, et al. 2021. Biosci Rep. 41:. PubMed Godoy-Calderón MJ, et al. 2018. Oncotarget. 8:11370. PubMed Wu Y, et al. 2019. Nat Commun. 10:58. PubMed Cornelison RC, et al. 2022. NPJ Precis Oncol. 6:54. PubMed Wang L, et al. 2020. Sci Rep. 10:16326. PubMed Collier AF, et al. 2018. J Pharmacol Exp Ther. 364:409. PubMed Choi SH, et al. 2020. Arterioscler Thromb Vasc Biol. :ATVBAHA120315485. PubMed Cushing EM, et al. 2018. J Lipid Res. 59:1230. PubMed Ikezoe T, et al. 2021. Biomolecules. 11:. PubMed Koch PS, et al. 2021. Front Physiol. 12:722394. PubMed Phua T, et al. 2017. Sci Rep. 10.1038\/srep44351. PubMed Cheng X, et al. 2021. Redox Biol. 40:101863. PubMed Milasan A, et al. 2019. Atherosclerosis. 283:106. PubMed Wong NR, et al. 2021. Immunity. 54:2072. PubMed Tsai MS, et al. 2021. Int J Mol Sci. 22:. PubMed Ni K, et al. 2018. Respir Res. 0.865972222. PubMed Dong Y, et al. 2021. STAR Protoc. 2:100853. PubMed Yue F, et al. 2021. Mol Ther. 29:132. PubMed Healy A, et al. 2020. Arterioscler Thromb Vasc Biol. 40:714. PubMed Kayama H, et al. 2012. Proc Natl Acad Sci U S A. 109:5010. PubMed Han X, et al. 2020. Mol Carcinog. 59:1088. PubMed\u003cbr\u003e\nRRID: AB_2044003 (BioLegend Cat. No. 137001) AB_2044004 (BioLegend Cat. No. 137002)\u003cbr\u003e\nStructure: A member of the lysosomal-associated membrane protein (lamp) family.\u003cbr\u003e\nDistribution: Expressed on tissue macrophages, Langerhans cells, and at low levels on dendritic cells.\u003cbr\u003e\nFunction: Involved in cell-cell interaction or cell-ligand interaction, still not completely understood.\u003cbr\u003e\nCell Type: Antigen-presenting cells, Dendritic cells, Langerhans cells, Leukocytes, Macrophages\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules, Innate Immune Signaling\u003cbr\u003e\nAntigen References: 1. Ramprasad MP, et al. 1996. Proc. Natl. Acad. Sci. USA 93:14833. 2. Smith MJ, et al. 1987. J. Cell. Sci. 87:113.\u003cbr\u003e\nGene ID: 12514\u003cbr\u003e\nUniProt: View information about CD68 on UniProt.org\u003cbr\u003e\nClone: FA-11\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Macrosialin\u003cbr\u003e\nIsotype: Rat IgG2a\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46863995044009,"sku":"137001","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-137001","provider":"Iright","version":"1.0","type":"link"}