{"product_id":"biolegend-138001","title":"Biolegend, 138001, Purified anti-mouse CD144 (VE-cadherin) Antibody, 50μg","description":"\u003cp\u003eCD144, also known as vascular endothelial-cadherin (VE-cadherin), is a 120 kD member of the type II Cadherin family.  It is an endothelial specific hemophilic adhesion molecule involved in endothelial cell survival, migration, contact-dependent growth inhibition, and homophilic adhesion.  VE-cadherin is essential for maintaining the integrity of the endothelial barrier in vivo .\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: VE-cadherin-Ig fusion protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested WB, ICC, Block - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. It is also recommended using EDTA-based solutions for dissociating attachment-dependent cell lines.\u003cbr\u003e\nApplication Notes: Clone BV13 recognizes an epitope between aa 45 and 56, and has a binding affinity of 5-15 nM5. Additional reported applications (for relevant formats) include: Western blotting1, blocking of cell interactions in vivo1, and immunofluorescence microscopy staining4.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Corada M, et al. 1999. P. Natl. Acad. Sci. USA 96:9815. (WB, Block) Liao F, et al. 2000. Cancer Res. 60:6805. (FC) Crosby CV, et al. 2005. Blood 105:2771. (FC) Liao F, et al. 2002. Cancer Res. 62:2567. (IF) May C, et al. 2005. Blood 105:4337. (epitope)\u003cbr\u003e\nProduct Citations: Gómez-Salinero JM, et al. 2022. Nat Cardiovasc Res. 1:882. PubMed Long Y, et al. 2022. Front Pharmacol. 13:1039103. PubMed Harrison CB, et al. 2020. Arterioscler Thromb Vasc Biol. ATVBAHA120315322:. PubMed Piao W, et al. 2020. Cell Reports. 30(4):1052-1062.e5.. PubMed Poulos MG, et al. 2017. J Clin Invest. 127:4163. PubMed Sakurai Y, et al. 2018. Mol Ther Oncolytics. 11:102. PubMed Wakabayashi T et al. 2018. Cell stem cell. 22(3):384-397 . PubMed Ecker BL, et al. 2019. Cancer Discov. 0.431944444. PubMed\u003cbr\u003e\nRRID: AB_10570497 (BioLegend Cat. No. 138001) AB_10569559 (BioLegend Cat. No. 138002)\u003cbr\u003e\nStructure: A 120 kD member of type II Cadherin family\u003cbr\u003e\nDistribution: Endothelial cells\u003cbr\u003e\nFunction: Involved in endothelial cell survival, migration, contact-dependent growth inhibition, and hemophilic adhesion\u003cbr\u003e\nLigand\/Receptor: CD144\u003cbr\u003e\nCell Type: Endothelial cells, Mesenchymal Stem Cells\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Allport JR, et al. 2002. J. Leukocyte Biol. 71:821. 2. Hirashima M, et al. 2009. Blood 93:1253. 3. Matsuyoshi N, et al. 1997. Proc. Assoc. Am. Physicians 109:362. 4. Matsumura K, et al. 2003. Blood 101:1367. 5. Hirashima M, et al. 2009. Blood 101:2261. 6. Gotsch U, et al. 1997. J. Cell Sci. 110:583. 7. Kataoka H, et al. 1997. Dev. Growth Differ. 39:729.\u003cbr\u003e\nGene ID: 12562\u003cbr\u003e\nUniProt: View information about CD144 on UniProt.org\u003cbr\u003e\nClone: BV13\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Vascular endothelial-cadherin\u003cbr\u003e\nIsotype: Rat IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862927102121,"sku":"138001","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-138001","provider":"Iright","version":"1.0","type":"link"}