{"product_id":"biolegend-138102","title":"Biolegend, 138102, Purified anti-mouse CD144 (VE-cadherin) Antibody, 500μg","description":"\u003cp\u003eCD144, also known as vascular endothelial-cadherin (VE-cadherin), is a 120 kD member of the type II Cadherin family.  It is an endothelial specific hemophilic adhesion molecule involved in endothelial cell survival, migration, contact-dependent growth inhibition and homophilic adhesion.  VE-cadherin is essential for maintaining the integrity of the endothelial barrier in vivo .\u003cbr\u003e\n500μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: VE-cadherin protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested IP, WB, ICC - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. It is also recommended using EDTA-based solutions for dissociating attachment-dependent cell lines.\u003cbr\u003e\nApplication Notes: Additional reported applications (for relevant formats) include: Immunoprecipitation1, Western Blot1, functional assay2, 3 by inhibiting vascular endothelial cell-cell interactions and immunocytochemistry staining of cultured cell lines4.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Allport JR, et al. 2002. J. Leukoc. Biol. 71:821. (IP WB) Hirashima M, et al. 2009. Blood 93:1253. (Block) Matsuyoshi N, et al. 1997. Proc. Assoc. Am. Physicians 109:362. (Block) Matsumura K, et al. 2003. Blood 101:1367. (IF) Hirashima M, et al. 2009. Blood 101:2261. (FC) Urbschat A, et al. 2014. PLoS One. 9:84432. PubMed\u003cbr\u003e\nProduct Citations: Brulois K, et al. 2020. Nat Commun. 3.095833333. PubMed Li GY, et al. 2023. J Pharm Anal. 13:262. PubMed Fischer D, et al. 2018. Front Pharmacol. 8:E10. PubMed Wang JC, et al. 2019. J Exp Clin Cancer Res. 38:235. PubMed Urbschat A, et al. 2014. PLoS One. 9:84432. PubMed Khaiboullina S, et al. 2019. Front Pharmacol. 10:642. PubMed\u003cbr\u003e\nRRID: AB_10569116 (BioLegend Cat. No. 138101)\u003cbr\u003e\nStructure: A 120 kD member of type II Cadherin family\u003cbr\u003e\nDistribution: Endothelial cells\u003cbr\u003e\nFunction: Involved in endothelial cell survival, migration, contact-dependent growth inhibition, and hemophilic adhesion.\u003cbr\u003e\nCell Type: Endothelial cells, Mesenchymal Stem Cells\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity, Stem Cells\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Gotsch U, et al. 1997. J. Cell Sci. 110:583. 2. Kataoka H, et al. 1997. Dev. Growth Differ. 39:729.\u003cbr\u003e\nGene ID: 12562\u003cbr\u003e\nUniProt: View information about CD144 on UniProt.org\u003cbr\u003e\nClone: VECD1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Vascular endothelial-cadherin, VE-cadherin\u003cbr\u003e\nIsotype: Rat IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864081584297,"sku":"138102","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-138102","provider":"Iright","version":"1.0","type":"link"}