{"product_id":"biolegend-139307","title":"Biolegend, 139307, PerCP\/Cyanine5.5 anti-mouse CD64 (FcγRI) Antibody, 25μg","description":"\u003cp\u003eCD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcRI. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes\/macrophages, dendritic cells, and mast cells. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG\/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: BALB\/c mouse FcγRI-human IgG Fc fusion protein.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP\/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: The X54-5\/7.1 antibody reacts with mouse strains carrying CD64a and b alleles but not CD64d. X54-5\/7.1 recognizes a conformational determinant formed between domains 2 and 3. Additional reported application (for relevant formats) include: immunoprecipitation1, and spatial biology (IBEX)5,6. Clone X54-5\/7.1 is not found to be useful for Western blots1.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Tan PS, et al. 2003. J. Immunol. 170:2549. (IP) Ingersoll MA, et al. 2010. Blood 115:e10. (FC) Ozeri E, et al. 2012. J. Immunol. 189:146. PubMed Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Gogoi H, et al. 2020. Front Immunol. 1.620833333. PubMed van Wijck Y, et al. 2018. J Immunol. 200:1570. PubMed Vaillancourt M, et al. 2023. PLoS Pathog. 19:e1010942. PubMed Maccio-Maretto L, et al. 2022. Immunology. 167:64. PubMed Mansouri S, et al. 2022. J Immunol. 209:2114. PubMed Caslin HL, et al. 2023. Front Immunol. 13:984859. PubMed Pham THM, et al. 2020. Cell Host \u0026amp; Microbe. 27(1):54-67.e5.. PubMed Pisu D, et al. 2020. STAR Protoc. 1:100123. PubMed De Schepper S et al. 2018. Cell. 175(2):400-415 . PubMed Dodagatta-Marri E, et al. 2021. Cell Rep. 36:109309. PubMed Marinaik CB, et al. 2020. Cell Rep Med. 1:100095. PubMed Baasch S, et al. 2021. Cell. . PubMed Linehan JL et al. 2018. Cell. 172(4):784-796 . PubMed Hubbard JJ, et al. 2020. J Exp Med. :217. PubMed Ahadome S, et al. 2016. JCI Insight. 1:e87012. PubMed Yadava K et al. 2019. Elife. 8 pii: e44821. PubMed Toomey CB, et al. 2018. Invest Ophthalmol Vis Sci. 59:662. PubMed Lee L, et al. 2016. PLoS One. 11:e0167693. PubMed Pisu D, et al. 2020. Cell Rep. 30:335. PubMed Mossadegh–Keller N, et al. 2019. Bio Protoc. 9:5. PubMed Lee W, et al. 2021. J Virol. 95:e0053021. PubMed Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed Shibata T, et al. 2020. J Clin Invest. 130:3021. PubMed Kolter J, et al. 2020. Immunity. 50(6):1482-1497. PubMed Sung PS, et al. 2022. JCI Insight. :. PubMed Heinen A, et al. 2019. Mol Ther. 27:46:00. PubMed Hakim R, et al. 2021. J Neurosci. 41:8441. PubMed Feuerstein R, et al. 2015. J Immunol. 194:2735. PubMed Kolter J, et al. 2016. J Immunol. 196: 2733 - 2741. PubMed Yang C, et al. 2019. J Transl Med. 1.004166667. PubMed Subramanian S, et al. 2022. Nat Immunol. 23:458. PubMed Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed Ge Y, et al. 2020. Mucosal Immunol. 13:34. PubMed Huang WC, et al. 2019. Int J Hyperthermia. 36:1255. PubMed\u003cbr\u003e\nRRID: AB_2561962 (BioLegend Cat. No. 139307) AB_2561963 (BioLegend Cat. No. 139308)\u003cbr\u003e\nStructure: Ig superfamily, type I glycoprotein, 72 kD\u003cbr\u003e\nDistribution: Monocytes, macrophages, mast cells, dendritic cells\u003cbr\u003e\nFunction: Phagocytosis, ADCC\u003cbr\u003e\nLigand\/Receptor: IgG\u003cbr\u003e\nCell Type: Dendritic cells, Macrophages, Mast cells, Monocytes\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: CD Molecules, Fc Receptors\u003cbr\u003e\nGene ID: 14129\u003cbr\u003e\nUniProt: View information about CD64 on UniProt.org\u003cbr\u003e\nClone: X54-5\/7.1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: FcRI\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864098492585,"sku":"139307","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-139307","provider":"Iright","version":"1.0","type":"link"}