{"product_id":"biolegend-139314","title":"Biolegend, 139314, PE\/Cyanine7 anti-mouse CD64 (FcγRI) Antibody, 100μg","description":"\u003cp\u003eCD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcRI. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes\/macrophages, dendritic cells, and mast cells. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG\/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: BALB\/c mouse FcγRI-human IgG Fc fusion protein.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE\/Cyanine7 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: The X54-5\/7.1 antibody reacts with mouse strains carrying CD64a and b alleles but not CD64d. X54-5\/7.1 recognizes a conformational determinant formed between domains 2 and 3. Additional reported application (for relevant formats) include: immunoprecipitation1, and spatial biology (IBEX)5,6. Clone X54-5\/7.1 is not found to be useful for Western blots1.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Tan PS, et al. 2003. J. Immunol. 170:2549. (IP) Ingersoll MA, et al. 2010. Blood 115:e10. (FC) Ozeri E, et al. 2012. J. Immunol. 189:146. PubMed Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed Gallizioli M, et al. 2020. Cell Rep. 33:108291. PubMed Zhang L, et al. 2022. Immunol Cell Biol. 100:691. PubMed Bohrer AC, et al. 2022. Cell Rep. 40:111144. PubMed Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed Chang CY, et al. 2022. Sci Adv. 8:eabq0615. PubMed Peng W, et al. 2022. Cell Mol Life Sci. 79:602. PubMed Zhao Q, et al. 2023. Signal Transduct Target Ther. 8:40. PubMed Larson-Casey JL, et al. 2023. JCI Insight. 8:. PubMed Pankhurst TE, et al. 2023. Cell Rep. 42:112310. PubMed Cohen K, et al. 2021. Cell Rep. 37:110026. PubMed Ozga AJ, et al. 2022. Immunity. 55:82. PubMed Zhou H, et al. 2022. EMBO Rep. 23:e53791. PubMed Akter S, et al. 2022. Cell Rep. 39:110983. PubMed Mugarza E, et al. 2022. Sci Adv. 8:eabm8780. PubMed Huseni MA, et al. 2023. Cell Rep Med. 4:100878. PubMed Draijer C, et al. 2023. Front Immunol. 13:1056477. PubMed Philpott J, et al. 2022. Immunohorizons. 6:366. PubMed Larson-Casey JL, et al. 2021. FASEB J. 35:e21675. PubMed Böttcher JP, et al. 2018. Cell. 172:1022. PubMed Larsen SE, et al. 2021. Sci Rep. 11:9040. PubMed Atkin-Smith G, et al. 2017. Sci Rep. 7:39846. PubMed Webster HC, et al. 2020. J Immunol Methods. 112702:477. PubMed Mitsune A, et al. 2021. Respir Res. 22:232. PubMed Helm M, et al. 2022. Life (Basel). 12:. PubMed Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed Antunes KH, et al. 2022. Front Immunol. 13:867022. PubMed Chen L, et al. 2020. J Exp Med. 217:00:00. PubMed Affo S, et al. 2021. Cancer Cell. 39(6):866-882.e11. PubMed Fantauzzi MF, et al. 2021. ERJ Open Res. 7: . PubMed Hole CR, et al. 2019. Nat Commun. 10:2955. PubMed Freemerman AJ, et al. 2019. J Immunol. 202:1265. PubMed Smith AS, et al. 2022. J Immunother Cancer. 10:. PubMed Alikhanyan K, et al. 2021. Cancers (Basel). 13:. PubMed Progatzky F, et al. 2021. Nature. 599:125. PubMed Yadav P, et al. 2021. J Immunol. 206:2888. PubMed Liu CY, et al. 2020. Cell Rep. 33:108275. PubMed Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed Wu Y, et al. 2021. PLoS Negl Trop Dis. 15:e0010050. PubMed Macdougall CE et al. 2018. Cell metabolism. 27(3):588-601 . PubMed Martínez‐López M et al. 2019. Immunity. 50(2):446-461 . PubMed Casulli J, et al. 2019. Nat Commun. 10:2121. PubMed Zheng J, et al. 2020. Nature. . PubMed Olson GS, et al. 2021. Cell Reports. 35(9):109195. PubMed Kaya B, et al. 2020. Cell Reports. 32(5):107979. PubMed Smith CA, et al. 2019. JCI Insight. 4. PubMed Luo J et al. 2018. Immunity. 49(1):107-119 . PubMed Maulloo CD, et al. 2021. Front Immunol. 12:714842. PubMed Larson-Casey JL, et al. 2021. J Biol Chem. :100810. PubMed Giampazolias E, et al. 2021. Cell. . PubMed Youshani AS, et al. 2019. J Neuroinflammation. 16:25. PubMed Murray MP, et al. 2022. Cell Rep. 38:110209. PubMed Chen F, et al. 2022. Cell Rep. 38:110215. PubMed Xu J, et al. 2020. Immunity. 53(2):371-383.e5. PubMed Birkl D, et al. 2019. FASEB J. 33:13632. PubMed Carlson PM, et al. 2021. J Immunol. 207:720. PubMed Wong NR, et al. 2021. Immunity. 54:2072. PubMed Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed Choi J, et al. 2020. Cell Stem Cell. 27:366. PubMed Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed Bosteels C, et al. 2020. Immunity. 52(6):1039-1056.e9.. PubMed Rochford I, et al. 2021. Am J Physiol Lung Cell Mol Physiol. 321:L686. PubMed Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed Larson–Casey JL, et al. 2019. J Clin Invest. 129:4962. PubMed Wang L, et al. 2020. J Nanobiotechnology. 18:38. PubMed Rappe JCF, et al. 2021. J Exp Med. 218:. PubMed\u003cbr\u003e\nRRID: AB_2563903 (BioLegend Cat. No. 139313) AB_2563904 (BioLegend Cat. No. 139314)\u003cbr\u003e\nStructure: Ig superfamily, type I glycoprotein, 72 kD\u003cbr\u003e\nDistribution: Monocytes, macrophages, mast cells, dendritic cells\u003cbr\u003e\nFunction: Phagocytosis, ADCC\u003cbr\u003e\nLigand\/Receptor: IgG\u003cbr\u003e\nCell Type: Dendritic cells, Macrophages, Mast cells, Monocytes\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: CD Molecules, Fc Receptors\u003cbr\u003e\nGene ID: 14129\u003cbr\u003e\nUniProt: View information about CD64 on UniProt.org\u003cbr\u003e\nClone: X54-5\/7.1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: FcRI\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864098328745,"sku":"139314","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-139314","provider":"Iright","version":"1.0","type":"link"}