{"product_id":"biolegend-139509","title":"Biolegend, 139509, PerCP\/Cyanine5.5 anti-mouse TCR Vβ5.1, 5.2 Antibody, 25μg","description":"\u003cp\u003eVβ5.1 and 5.2 T cell receptor (TCR Vβ5.1, 5.2) are variants of TCR β chain that, along with TCR α chain, forms the TCR heterodimer. In association with the CD3 complex, TCR α\/β is responsible for antigen recognition in the MHC-Peptide complex and the initiation of T cell-mediated immune responses.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Murine T cell hybridoma 2HB51.8\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP\/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: Induction of proliferation of Vß5.1+ and Vß5.2+ T cells2, 3 and in vivo depletion of Vß5+ T cells4.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Kanagawa O, et al. 1991. J. Immunol. 147:1307. (FC) Kanagawa O, et al. 1992. J. Immunol. 149:9. (Activ) Woodland DL, et al. 1993. J. Exp. Med. 177:433. (Activ) Gelber C, et al. 1992. Cancer Res. 52:6507. (Deplete)\u003cbr\u003e\nProduct Citations: Chien CH, et al. 2024. STAR Protoc. 103136:5. PubMed Castellanos CA, et al. 2021. Sci Immunol. 6:eabh0707. PubMed\u003cbr\u003e\nRRID: AB_2566806 (BioLegend Cat. No. 139509) AB_2566807 (BioLegend Cat. No. 139510)\u003cbr\u003e\nStructure: Member of the immunoglobulin superfamily, Vβ5.1and 5.2 are variants of the TCR β chain which associates with the TCR α chain to form the TCR heterodimer.\u003cbr\u003e\nDistribution: Expressed on a subset of TCR αβ+ T cells.\u003cbr\u003e\nFunction: Recognition of peptides presented by the MHC molecules, responsible for T cell mediated immune responses.\u003cbr\u003e\nLigand\/Receptor: MHC-Peptide complex\u003cbr\u003e\nCell Type: T cells\u003cbr\u003e\nBiology Area: Adaptive Immunity, Immunology\u003cbr\u003e\nMolecular Family: TCRs\u003cbr\u003e\nAntigen References: 1. Marrack P, et al. 2008. Annu. Rev. Immunol. 26:171. 2. Sim GK and Augustin AA. 1985. Cell 42:89. 3. Mami-Chouaib F, et al. 2002. Immunol. Rev. 188:114.\u003cbr\u003e\nGene ID: 21577\u003cbr\u003e\nUniProt: View information about TCR Vbeta5.1 5.2 on UniProt.org\u003cbr\u003e\nClone: MR9-4\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Vβ5 T-cell receptor, T cell receptor beta 5\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866153799849,"sku":"139509","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-139509","provider":"Iright","version":"1.0","type":"link"}