{"product_id":"biolegend-140321","title":"Biolegend, 140321, PerCP\/Cyanine5.5 anti-mouse CD90.2 (Thy1.2) Antibody, 25μg","description":"\u003cp\u003eCD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy1.2, a GPI-linked membrane molecule. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb\/c, CBA\/J, C3H\/He, C57BL\/-, DBA, NZB\/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction of hematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse thymus or spleen\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP\/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining1 of frozen tissue section, immunofluorescence2, and immunoprecipitation3. Does not react with Thy-1.1 (CD90.1).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Aldrich M, et al. 2003. J. Immunol. 171:5562. (IHC) Jameson J, et al. 2004. J. Immunol. 172:3573. (IF) Okada C, et al. 1990. J. Immunol. 144:3473. (IP)\u003cbr\u003e\nProduct Citations: Logan K Smith et al. 2018. Immunity. 48(2):299-312 . PubMed Dragan M, et al. 2022. J Invest Dermatol. 142:583. PubMed Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed Wang X, et al. 2020. Signal Transduct Target Ther. 5:35. PubMed Mathur S, et al. 2021. JCI Insight. 6:. PubMed Boothby IC, et al. 2021. Nature. 599:667. PubMed Ku C, et al. 2015. Genes Dev. 29: 1930-1941. PubMed Kolev M, et al. 2020. Immunity. 52(3):513-527. PubMed Ko HJ, et al. 2020. Front Immunol. 1897:11. PubMed Wang D, et al. 2022. EMBO Rep. 23:e53691. PubMed Dallari S, et al. 2021. Cell Host Microbe. 29(6):1014-1029.e8. PubMed Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed Lal JC, et al. 2021. Breast Cancer Res. 23:83. PubMed\u003cbr\u003e\nRRID: AB_2562695 (BioLegend Cat. No. 140321) AB_2562696 (BioLegend Cat. No. 140322)\u003cbr\u003e\nStructure: Ig superfamily, 25-35 kD\u003cbr\u003e\nDistribution: Hematopoietic stem cells and neurons, epithelial cells, fibroblasts, neurons, all thymocytes, and peripheral T cells of Thy-1.2 bearing mice\u003cbr\u003e\nFunction: Lymphocyte costimulation, proliferation, and differentiation of hematopoietic stem cells\u003cbr\u003e\nLigand\/Receptor: CD45\u003cbr\u003e\nCell Type: Epithelial cells, Fibroblasts, Hematopoietic stem and progenitors, Neurons, T cells, Thymocytes\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Immunology, Signal Transduction\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Borrello M, et al. 1996. Cell. Immunol.173:198. 2. Radrizzani M, et al. 1995. J. Neurosci. Res. 42:220. 3. Williams A, et al. 1982. Science 216:696.\u003cbr\u003e\nGene ID: 21838\u003cbr\u003e\nUniProt: View information about CD90.2 on UniProt.org\u003cbr\u003e\nClone: 53-2.1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Thy-1.2\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862430208169,"sku":"140321","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-140321","provider":"Iright","version":"1.0","type":"link"}