{"product_id":"biolegend-140421","title":"Biolegend, 140421, APC\/Cyanine7 anti-mouse CD8b.2 Antibody, 25μg","description":"\u003cp\u003eCD8b is the 32 kD β chain of CD8, also known as Lyt-3.2 or Ly-3.2. It is a member of the Ig superfamily expressed as a heterodimer with the CD8α chain on a subset of MHC class I-restricted T cells and most thymocytes. CD8 is a co-receptor for the TCR complex involved in T cell activation. The antibody 53-5.8 is specific for Ly-3.2 and has low reactivity with Ly-3.1.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse thymus or spleen\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with APC\/Cyanine7 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunofluorescence3, immunohistochemical staining4 of frozen tissue section using dry ice-isopentane method and immunoprecipitation5.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Ledbetter J, et al. 1979. Immunol. Rev. 47:63. Ledbetter J, et al. 1980. J. Exp. Med. 152:280. Vremec D, et al. 2000. J. Immunol. 164:2978. (IF) Lawrence D, et al. 1999. J. Virol. 73:1795. (IHC) Bosselut R, et al. 1999. J. Exp. Med. 190:1517. (IP)\u003cbr\u003e\nProduct Citations: Molitoris KH, et al. 2024. JBMR Plus. ziae023:8. PubMed\u003cbr\u003e\nRRID: AB_2860690 (BioLegend Cat. No. 140421) AB_2876483 (BioLegend Cat. No. 140422)\u003cbr\u003e\nStructure: Ig superfamily, CD8β chain, 32 kD\u003cbr\u003e\nDistribution: Majority of thymocytes, cytotoxic T cell subsets\u003cbr\u003e\nFunction: Co-receptor of TCR\u003cbr\u003e\nLigand\/Receptor: MHC class I\u003cbr\u003e\nCell Type: T cells, Thymocytes\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, TCRs\u003cbr\u003e\nAntigen References: 1. Ledbetter J, et al. 1981. J. Exp. Med. 153:1503. 2. Renard V, et al. 1996. J. Exp. Med. 184:2439.\u003cbr\u003e\nGene ID: 12526\u003cbr\u003e\nUniProt: View information about CD8beta.2 on UniProt.org\u003cbr\u003e\nClone: 53-5.8\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Lyt-3.2, Ly-3.2\u003cbr\u003e\nIsotype: Rat IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862431027369,"sku":"140421","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-140421","provider":"Iright","version":"1.0","type":"link"}