{"product_id":"biolegend-141209","title":"Biolegend, 141209, PE\/Cyanine7 anti-mouse CD133 Antibody, 25μg","description":"\u003cp\u003eCD133 (also known as prominin-1 and AC133) is a 120 kD pentaspan glycoprotein with 5 transmembrane domains which localize to cellular protrusions. CD133 was initially described as a surface antigen specific for human hematopoietic stem cells and as a marker for murine neuroepithelial cells and some embryonic epithelia. Later on, CD133 was found on other stem cells, including endothelial progenitor cells, glioblastomas, neuronal, and glial stem cells. In addition to hematopoietic stem cells, CD133 was found on cancer cells, such as some leukemia cells and brain tumor cells. It also has been detected at low levels in the kidney, pancreas, placenta, and fetal liver tissue. Although the biological function of CD133 is not completely understood, CD133 has been extensively used as a stem cell marker for normal and cancerous tissues.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: CD133-expressing-GFP-RBL2H3 plus CFA as adjuvant.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE\/Cyanine7 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Zhang Y, et al. 2012. Diabetes. 61:2114. PubMed\u003cbr\u003e\nProduct Citations: Koizumi M, et al. 2022. FASEB J. 36:e22662. PubMed Harada Y, et al. 2021. Nat Commun. 12:6562. PubMed Zhou X, et al. 2017. J Vasc Surg. 65:1161. PubMed Tsuboi M, et al. 2018. Dev Cell. 47:758. PubMed Raman S, et al. 2021. Cell Reports Medicine. 2(7):100344. PubMed\u003cbr\u003e\nRRID: AB_2564068 (BioLegend Cat. No. 141209) AB_2564069 (BioLegend Cat. No. 141210)\u003cbr\u003e\nStructure: 120 kD pentaspan transmembrane glycoprotein\u003cbr\u003e\nDistribution: Hematopoietic stem cells, epithelial progenitor cells, leukemia cells, brain tumor cells, kidney, pancreas, placenta, and fetal liver cells\u003cbr\u003e\nFunction: Biological function unknown\u003cbr\u003e\nCell Type: Epithelial cells, Hematopoietic stem and progenitors, Leukemia, Neural Stem Cells\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Singh SK, et al. 2004. Nature 18:396. 2. Falini B, et al. 2005. New Engl. J. Med. 352:254. 3. Bonanno G, et al. 2004. Transfusion 44:1087. 4. Gaipa G, et al. 2002. Haematologica 87:347. 5. Wuchter C, et al. 2001. Haematologica 86:154.\u003cbr\u003e\nGene ID: 19126\u003cbr\u003e\nUniProt: View information about CD133 on UniProt.org\u003cbr\u003e\nClone: 315-2C11\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Prominin-1, AC133\u003cbr\u003e\nIsotype: Rat IgG2a, λ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862366965929,"sku":"141209","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-141209","provider":"Iright","version":"1.0","type":"link"}