{"product_id":"biolegend-141607","title":"Biolegend, 141607, PE\/Cyanine7 anti-mouse H-2K, 25μg","description":"\u003cp\u003eThis antibody has been proven to be very useful in tracking the quantity and localization of these specific antigen-presenting cells (APC) in vivo .\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: SIINFEKL pulsed RMA-S cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE\/Cyanine7 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: The 25-D1.16 monoclonal antibody specifically reacts with ovalbumin-derived peptide SIINFEKL bound to H-2Kb of MHC class I, but not with unbound H-2Kb or H-2Kb bound with an irrelevant peptide. Additional reported applications (for relevant formats) include: immunofluorescence microscopy2,3, immunohistochemical staining of frozen tissue sections3 , and inhibition of T cell response to H-2Kb-SIINFEKL in vitro.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Mareeva T, et al. 2010. J. Immunol. Methods 353:78. Dolan BP, et al. 2010. J. Immunol. 184:1419. (IF) Porgador A, et al. 1997. Immunity 6:715. (IF, IHC) Herve J, et al. 2013. J. Immunol. 190:3163. PubMed.\u003cbr\u003e\nProduct Citations: Arora H, et al. 2019. Immunity. 50:418. PubMed Drijvers JM, et al. 2021. Cancer Immunol Res. 9:184. PubMed Liu X, et al. 2020. Nature. . PubMed Baasch S, et al. 2021. Cell. . PubMed Zhao X, et al. 2022. Nat Protoc. 17:2240. PubMed Mainini F, et al. 2015. PLoS One. 10: 0145403. PubMed Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed Gammon J, et al. 2015. J Control Release. 210: 169-178. PubMed\u003cbr\u003e\nRRID: AB_11219193 (BioLegend Cat. No. 141607) AB_11218593 (BioLegend Cat. No. 141608)\u003cbr\u003e\nStructure: OVA peptide257-264 and H-2Kb complex\u003cbr\u003e\nDistribution: SIINFEKL bond H-2Kb bearing cells\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: MHC Antigens\u003cbr\u003e\nAntigen References: 1. Mareeva T, et al. 2010. J. Immunol. Methods 353:78. 2. Mareeva T, et al. 2008. J. Biol. Chem. 283:29053. 3. Deng Y, et al. 1998. J. Immunol. 161:1677.\u003cbr\u003e\nGene ID: 14972\u003cbr\u003e\nUniProt: View information about H-2Kb bound to SIINFEKL on UniProt.org\u003cbr\u003e\nClone: 25-D1.16\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: MHC class I molecule Kb bound to the peptide SIINFEKL (Kb-SIINFEKL) H-2Kb, OVA agonist peptide (OVAp), OVA-Kb, OVA257-264 (SIINFEKL)-H-2kb, OVA257-264-H-2kb\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860879954089,"sku":"141607","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-141607","provider":"Iright","version":"1.0","type":"link"}