{"product_id":"biolegend-141716","title":"Biolegend, 141716, PerCP\/Cyanine5.5 anti-mouse CD206 (MMR) Antibody, 100μg","description":"\u003cp\u003eCD206, also known as mannose receptor (MR), is a 175 kD type I membrane protein. It is a pattern recognition receptor (PRR) belonging to the C-type lectin superfamily. MR is expressed on macrophages, dendritic cells, Langerhans cells, and hepatic or lymphatic endothelial cells. MR recognizes a range of microbial carbohydrates bearing mannose, fucose, or N-acetyl glucosamine through its C-type lectin-like carbohydrate recognition domains, sulfated carbohydrate antigens through its cysteine-rich domain, and collagens through its fibronectin type II domain. MR mediates endocytosis and phagocytosis as well as activation of macrophages and antigen presentation. It plays an important role in host defense and provides a link between innate and adaptive immunity. Recently, MR on lymphatic endothelial cells was found to be involved in leukocyte trafficking and a contributor to the metastatic behavior of cancer cells. It suggests that MR may be a potential target in controlling inflammation and cancer metastasis by targeting the lymphatic vasculature.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Recombinant mouse CD206 (MMR)\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality testedFC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP\/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Clone C068C2 recognizes a region similar to clone MR5D3, based on the ability of the clones to block each other. Additional reported applications (for the relevant formats) include: spatial biology (IBEX)4,5.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Keller J, et al. 2012. Biochem Biophys Res Commun. 417:217. PubMed Ito H, et al. 2012. J Am Soc Nephrol. 23:1797. PubMed Yang X, et al. 2015. PNAS. 112:2900. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Lin C, et al. 2020. Cancer Immunol Res. 632:8. PubMed Choconta JL, et al. 2023. Immun Ageing. 20:22. PubMed Wang K, et al. 2023. Nat Commun. 14:2950. PubMed Yang C, et al. 2022. Nat Commun. 13:3426. PubMed Chen G, et al. 2021. J Mol Cell Cardiol. 159:62. PubMed Dolfi B, et al. 2022. Cell Rep. 39:110949. PubMed Gong F, et al. 2023. Adv Healthc Mater. 12:e2201771. PubMed Morfoisse F, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1346. PubMed Wang S, et al. 2022. Front Immunol. 13:869061. PubMed Zhao J, et al. 2022. J Nanobiotechnology. 20:62. PubMed Yerneni SS, et al. 2021. MBio. 12:e0165721. PubMed An YA et al. 2017. eLife. 6 pii: e24071. PubMed Wang C, et al. 2021. Cell Rep. 37:110021. PubMed Ano Y, et al. 2017. J Biol Chem. 292:3720. PubMed Gao H, et al. 2021. Cells. 10:. PubMed Gozgit JM, et al. 2021. Cancer Cell. :. PubMed Peng K, et al. 2017. PLoS One.. 10.1371\/journal.pone.0183271. PubMed Barsoumian HB, et al. 2020. J Immunother Cancer. 8:00. PubMed De Santi C, et al. 2022. Mol Ther Nucleic Acids. 29:643. PubMed Uddin MJ, et al. 2022. Mucosal Immunol. 15:165. PubMed Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed Bronisz–Budzynska I, et al. 2019. Skelet Muscle. 9:22. PubMed Xiao Y, et al. 2022. Nat Commun. 13:758. PubMed Shigeta A, et al. 2019. Dev Cell. 48:617. PubMed Chao Y, et al. 2020. Sci Adv. 6:eaaz4204. PubMed Zhang Z, et al. 2020. Front Immunol. 11:583276. PubMed Yang C, et al. 2019. J Transl Med. 1.004166667. PubMed Calvente CJ, et al. 2019. J Clin Invest. 130:4091. PubMed Zhou Y, et al. 2022. Theranostics. 12:5488. PubMed Barsoumian HB, et al. 2022. Cancers (Basel). 14:. PubMed Li Z, et al. 2022. Evid Based Complement Alternat Med. 2022:7421265. PubMed Wang C, et al. 2020. Mucosal Immunol. 13:22. PubMed Zhang J, et al. 2020. Cell Metabolism. 31(6):1136-1153. PubMed Cortez MA, et al. 2020. Nat Commun. 3.819444444. PubMed\u003cbr\u003e\nRRID: AB_2561991 (BioLegend Cat. No. 141715) AB_2561992 (BioLegend Cat. No. 141716)\u003cbr\u003e\nStructure: Type I transmembrane protein, 175 kD, C-type lectin superfamily\u003cbr\u003e\nDistribution: Macrophages, dendritic cells, Langerhans cells, liver endothelial cells\u003cbr\u003e\nFunction: Pathogen recognition, endocytosis and phagocytosis, antigen presentation\u003cbr\u003e\nLigand\/Receptor: Antigen containing mannose, fucose, or an N-acetyl glucosamine\u003cbr\u003e\nCell Type: Dendritic cells, Endothelial cells, Langerhans cells, Macrophages\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Signal Transduction\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Wileman TE, et al. 1986. P. Natl. Acad. Sci. USA 83:2501. 2. Apostolopoulos V, et al. 2001. Curr. Mol. Med. 1:469. 3. Burgdorf S, et al. 2006. J. Immunol. 176:6770. 4. McKenzie EJ, et al. 2007. J. Immunol. 178:4975.\u003cbr\u003e\nGene ID: 17533\u003cbr\u003e\nUniProt: View information about CD206 on UniProt.org\u003cbr\u003e\nClone: C068C2\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: MMR (macrophage mannose receptor), MR (mannose receptor), MRC1\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: Why is mouse CD206 stained intracellularly and not via surface staining?\u003cbr\u003e\nA: Typically, mouse CD206 surface level is relatively low under normal conditions and so intracellular staining protocol is required to get better signal.\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866086363305,"sku":"141716","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-141716","provider":"Iright","version":"1.0","type":"link"}