{"product_id":"biolegend-141809","title":"Biolegend, 141809, Brilliant Violet 421™ anti-mouse Allergin-1 Antibody, 50μg","description":"\u003cp\u003eAllergin-1, also known as mast cell Ag-32, is a 60 kD single pass transmembrane protein. It contains one Ig-like C2-type (immunoglobulin-like) domain in the extracellular region and one immunoreceptor tyrosine-based inhibitory motif (ITIM)-like domain in the cytoplasmic region. Allergin-1 is expressed on mast cells, dendritic cells, and macrophages as well as at low levels on neutrophils. After tyrosine phosphorylation, allergin-1 recruits PTPN6, PTPN11, and INPP5D, which results in a diminution of IgE-mediated degranulation in mast cells. No ligands have been identified for this molecule.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Transfected cells expressing allergin-1-Flag\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450\/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: Inhibits FcεRI-mediated degranulation in bone marrow-derived cultured mast cells1. Additional reported applications (for the relevant formats) include: blocking1.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Hitomi K, et al. 2010. Nat. Immunol. 11:601. (Block)\u003cbr\u003e\nRRID: AB_3674992 (BioLegend Cat. No. 141809)\u003cbr\u003e\nStructure: Single pass transmembrane protein of 60 kD; contains one Ig-like C2-like domain and one ITIM-like domain\u003cbr\u003e\nDistribution: Mast cells, dendritic cells, macrophages, neutrophils\u003cbr\u003e\nFunction: Inhibits IgE-mediated degranulation of mast cells\u003cbr\u003e\nInteraction: PTPN6, PTPN11 and INPP5D\u003cbr\u003e\nCell Type: Dendritic cells, Macrophages, Mast cells, Neutrophils\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Signal Transduction\u003cbr\u003e\nAntigen References: 1. Hitomi K, et al. 2010. Nat. Immunol. 11:601. 2. Pirozzi G, et al. 1995. J. Immunol. 155:5811.\u003cbr\u003e\nGene ID: 380732\u003cbr\u003e\nUniProt: View information about Allergin-1 on UniProt.org\u003cbr\u003e\nClone: TX83\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Allergy inhibitory receptor 1, mast cell Ag-32, MCA-32, Milr1\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What is the F\/P ratio range of our BV421™ format antibody reagents?\u003cbr\u003e\nA: It is lot-specific. On average it ranges between 2-4.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864061169833,"sku":"141809","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-141809","provider":"Iright","version":"1.0","type":"link"}