{"product_id":"biolegend-142402","title":"Biolegend, 142402, Purified anti-mouse CD169 (Siglec-1) Antibody, 100μg","description":"\u003cp\u003eCD169, also known as Siglec-1 and Sialoadhesin (Sn), is a type I lectin containing 17 immunoglobulin (Ig) domains (one variable domain and 16 constant domains). CD169 binds to sialic acids, which can be found on PSGL-1, CD43, CD206, and CD227. By its affinity to α2, 3-linked sialic acid, it is involved in macrophage binding to different cell types such as granulocytes, monocytes, NK, B, and T cells. CD169 was initially identified as a sialic acid-dependent sheep erythrocyte receptor (SER) on resident bone marrow cells of mice. It has been identified as highly expressed on resident bone marrow macrophages which plays an important role in retention of stem cells in mesenchymal stem cell niche. It is also found on some specific subsets of tissue macrophages in spleen, lymph nodes, bone marrow, liver, colon, lungs, and cancer cells. Evidence suggest that CD169-positive macrophages serve as lymph node-resident APCs to dominate early activation of tumor antigen-specific CD8 + T cells and invariant NK cell.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Purified Native Sialoadhesin from spleen\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested IHC-F - Verified SB - Community verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining in frozen tissue sections1, and spatial biology (IBEX)4,5.\u003cbr\u003e\nAdditional Product Notes: This product has been verified for IHC-F (Immunohistochemistry - frozen tissue sections) on the NanoString GeoMx® Digital Spatial Profiler. The GeoMx® enables researchers to perform spatial analysis of protein and RNA targets in FFPE and fresh frozen human and mouse samples. For more information about our spatial biology products and the GeoMx® platform, please visit our spatial biology page.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Barral P, et al. 2010. Nat. Immunol. 11:303. (IHC-F) Chtanova T, et al. 2008. Immunity 29:487. Klass M, et al. 2012. J. Immunol. 189:2414. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Marcial-Juárez E, et al. 2023. iScience. 26:106310. PubMed Rajagopalan A, et al. 2021. Cell Rep Methods. 1:. PubMed Blériot C, et al. 2020. Methods Mol Biol. 2164:87. PubMed Fernández-García V, et al. 2022. Biomed Pharmacother. 148:112769. PubMed Yu YA, et al. 2020. The Journal of Immunology. 204(6):1474-1485. PubMed Klaas M, et al. 2012. J Immunol. 189:2414. PubMed Hilligan KL, et al. 2020. Nat Commun. 11:5637. PubMed Mrdjen D et al. 2018. Immunity. 48(2):380-395 . PubMed Enders M, et al. 2020. J Immunol. 204:87. PubMed Blriot C, et al. 2021. Immunity. :. PubMed Jordan S, et al. 2020. Cell. 178(5):1102-1114.e17.. PubMed Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed Bajwa A, et al. 2015. J Am Soc Nephrol. 101681\/ASN2015010095. PubMed\u003cbr\u003e\nRRID: AB_10915134 (BioLegend Cat. No. 142401) AB_10916523 (BioLegend Cat. No. 142402)\u003cbr\u003e\nStructure: Type I single membrane-spanning lectin containing 17 immunoglobulin (Ig) domains, belongs to the immunoglobulin superfamily.\u003cbr\u003e\nDistribution: Macrophages in spleen, lymph nodes, bone marrow, liver, colon and lungs.\u003cbr\u003e\nFunction: Adhesion.\u003cbr\u003e\nLigand\/Receptor: PSGL-1, CD43, CD206 and CD227.\u003cbr\u003e\nCell Type: Macrophages\u003cbr\u003e\nBiology Area: Cell Biology, Immunology\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules, Protein Kinases\/Phosphatase, Siglec Molecules\u003cbr\u003e\nAntigen References: 1. Chow A, et al. 2011. J. Exp. Med. 208:261. 2. Asano K, et al. 2011. Immunity 34:85. 3. Xiong YS, et al. 2009. Clin. Biochem. 42:1057. 4. Varki A, et al. 2009. Glycoconj. J. 26:231. 5. Rempel H, et al. 2008. PLoS One 3:e1967. 6. Crocker PR, et al. 2001. Trends Immunol. 22:337. 7. Hartnell A, et al. 2001. Blood 97:288. 8. Crocker PR, et al. 1985. J. Exp. Med. 162:993.\u003cbr\u003e\nGene ID: 20612\u003cbr\u003e\nUniProt: View information about CD169 on UniProt.org\u003cbr\u003e\nClone: 3D6.112\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Sialic acid binding Ig-like lectin 1 (Siglec-1), Sialoadhesin (Sn)\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: If an antibody clone has been previously successfully used in IBEX in one fluorescent format, will other antibody formats work as well?\u003cbr\u003e\nA: It’s likely that other fluorophore conjugates to the same antibody clone will also be compatible with IBEX using the same sample fixation procedure. Ultimately a directly conjugated antibody’s utility in fluorescent imaging and IBEX may be specific to the sample and microscope being used in the experiment. Some antibody clone conjugates may perform better than others due to performance differences in non-specific binding, fluorophore brightness, and other biochemical properties unique to that conjugate.\u003cbr\u003e\nQ: Will antibodies my lab is already using for fluorescent or chromogenic IHC work in IBEX?\u003cbr\u003e\nA: Fundamentally, IBEX as a technique that works much in the same way as single antibody panels or single marker IF\/IHC. If you’re already successfully using an antibody clone on a sample of interest, it is likely that clone will have utility in IBEX. It is expected some optimization and testing of different antibody fluorophore conjugates will be required to find a suitable format; however, legacy microscopy techniques like chromogenic IHC on fixed or frozen tissue is an excellent place to start looking for useful antibodies.\u003cbr\u003e\nQ: Are other fluorophores compatible with IBEX?\u003cbr\u003e\nA: Over 18 fluorescent formats have been screened for use in IBEX, however, it is likely that other fluorophores are able to be rapidly bleached in IBEX. If a fluorophore format is already suitable for your imaging platform it can be tested for compatibility in IBEX.\u003cbr\u003e\nQ: The same antibody works in one tissue type but not another. What is happening?\u003cbr\u003e\nA: Differences in tissue properties may impact both the ability of an antibody to bind its target specifically and impact the ability of a specific fluorophore conjugate to overcome the background fluorescent signal in a given tissue. Secondary stains, as well as testing multiple fluorescent conjugates of the same clone, may help to troubleshoot challenging targets or tissues. Using a reference control tissue may also give confidence in the specificity of your staining.\u003cbr\u003e\nQ: How can I be sure the staining I’m seeing in my tissue is real?\u003cbr\u003e\nA: In general, best practices for validating an antibody in traditional chromogenic or fluorescent IHC are applicable to IBEX. Please reference the Nature Methods review on antibody based multiplexed imaging for resources on validating antibodies for IBEX.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862393245865,"sku":"142402","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-142402","provider":"Iright","version":"1.0","type":"link"}