{"product_id":"biolegend-143303","title":"Biolegend, 143303, PE anti-mouse Trem-like 4 (Treml4) Antibody, 25μg","description":"\u003cp\u003eTrem-like 4, a type I transmembrane protein and a member of the TREM family, consists of a single extracellular Ig-like domain of the V-type, a transmembrane domain, and a short cytoplasmic domain. Trem-like 4 is expressed on macrophages in the spleen, and dendritic cells, especially CD8+ dendritic cells. It is proposed to regulate the immune response based on its expression profile. It has been reported that B7-H3 is a receptor and that the soluble form of Treml4 has a binding affinity to late apoptotic or necrotic cells.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Extracellular domain of recombinant mouse Trem-like 4\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunofluorescent staining, immunoprecipitation, and Western blotting1.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Hemmi H, et al. 2009. J. Immunol. 182:1278. (WB)\u003cbr\u003e\nProduct Citations: Slusarczyk P, et al. 2023. Elife. 12: . PubMed Devalaraja S, et al. 2020. Cell. 1098:180. PubMed Pek RH, et al. 2019. Elife. 8:e49503. PubMed Alam Z, et al. 2020. Cell Rep. 107825:31. PubMed Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed\u003cbr\u003e\nRRID: AB_11126143 (BioLegend Cat. No. 143303) AB_11125372 (BioLegend Cat. No. 143304)\u003cbr\u003e\nStructure: Type I transmembrane protein; member of the TREM family\u003cbr\u003e\nDistribution: CD8+ dendritic cells, dendritic cell subsets, macrophages in the spleen, myeloid cells\u003cbr\u003e\nFunction: Regulates innate immune response and homeostatis\u003cbr\u003e\nInteraction: Associates with DAP12; binds late apoptotic cells\/necrotic cells\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Macrophages\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: MHC Antigens\u003cbr\u003e\nAntigen References: 1. Ford JW and McVicar DW. 2009. Curr. Opin. Immunol. 21:38. 2. Hemmi H, et al. 2009. J. Immunol. 182:1278.\u003cbr\u003e\nGene ID: 224840\u003cbr\u003e\nUniProt: View information about Trem-like 4 on UniProt.org\u003cbr\u003e\nClone: 16E5\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Trem-l4, TREM like transcript 4, triggering receptor expressed on myeloid cells-like 4\u003cbr\u003e\nIsotype: Rat IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860819955881,"sku":"143303","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-143303","provider":"Iright","version":"1.0","type":"link"}