{"product_id":"biolegend-145304","title":"Biolegend, 145304, PE anti-mouse IL-33Rα (IL1RL1, ST2) Antibody, 100μg","description":"\u003cp\u003eIL-33Rα, also known as ST2 or IL-1RL1, is a member of the Toll\/IL-1 receptor family.  It binds IL-33 and is structurally similar to IL-1R1.  Two forms of the protein exist - a soluble form known as ST2 and a membrane anchored form known as ST2L.  The membrane form is expressed by Th2 cells and bone marrow derived mast cells, whereas the soluble form is expressed by serum-stimulated fibroblasts.  Blocking with anti-ST2 antibodies has been shown to alleviate experimental arthritis and airway inflammation.  The IL-33-ST2 axis has been reported to be important across a range of diseases including asthma, allergies, and cardiac disease.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: IL-33Rα-hFcγ1 fusion protein.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Hashiguchi M, et al. 2014. Eur. J. Immunology. (FC) PubMed\u003cbr\u003e\nProduct Citations: Furuhashi K, et al. 2017. Immunology. 151:227. PubMed Koenig A, et al. 2022. Front Immunol. 12:791100. PubMed Dragan M, et al. 2022. J Invest Dermatol. 142:583. PubMed Karagiannis F, et al. 2020. Immunity. 52(4):620-634. PubMed Wang L, et al. 2019. Cell Rep. 29:1848. PubMed Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed Shani O, et al. 2020. Cancer Res. 80:5317. PubMed She L, et al. 2021. JCI Insight. 6:e143509. PubMed Wu G, et al. 2020. Br J Pharmacol. 177:5063. PubMed Wiesner D, et al. 2015. PLoS Pathog. 11:1004701. PubMed He J, et al. 2020. Cell Reports. 29(9):2718-2730.e6.. PubMed Nakajima T, et al. 2021. J Exp Med. 218:. PubMed Xi‐Zhi J Guo et al. 2018. Immunity. 49(3):531-544 . PubMed Klein O, et al. 2019. J Allergy Clin Immunol. 144:1074. PubMed Zhang M, et al. 2017. Mol Med Rep. 15:4291. PubMed Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed Dutton EE, et al. 2018. Wellcome Open Res. 2:117. PubMed Kastenschmidt JM, et al. 2021. Cell Rep. 35:108997. PubMed\u003cbr\u003e\nRRID: AB_2561914 (BioLegend Cat. No. 145303) AB_2561915 (BioLegend Cat. No. 145304)\u003cbr\u003e\nStructure: 63 kD, IL-1 receptor family member, single pass type I membrane protein\u003cbr\u003e\nDistribution: Mast cells, Th2 cells, and fibroblasts\u003cbr\u003e\nFunction: Following interaction with IL-33, it recruits MyD88, Irak1, Irak4 and Traf6 followed by phosphorylation of the MAP\/ERK pathway.\u003cbr\u003e\nInteraction: Interacts with MyD88, Irak1, Irak4 and Traf6\u003cbr\u003e\nLigand\/Receptor: IL-33\u003cbr\u003e\nCell Type: Fibroblasts, Mast cells, Th2\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: Cytokine\/Chemokine Receptors\u003cbr\u003e\nAntigen References: 1. Yanagisawa K, et al. 1993. FEBS Lett. 318:83. 2. Schmitt E, et al. 1990. Cytokine 6:407. 3. Yanagisawa K, et al. 1992. FEBS Lett. 302:51. 4. Takagi T, et al. 1993. Biochim Biophys Acta. 1178:194.\u003cbr\u003e\nGene ID: 17082\u003cbr\u003e\nUniProt: View information about IL-33Ralpha on UniProt.org\u003cbr\u003e\nClone: DIH9\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: T1\/ST2, ST2L, IL-1R1, T1, DER4, IL1R1\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46863010332841,"sku":"145304","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-145304","provider":"Iright","version":"1.0","type":"link"}