{"product_id":"biolegend-145512","title":"Biolegend, 145512, Brilliant Violet 421™ anti-mouse CD185 (CXCR5) Antibody, 500microl","description":"\u003cp\u003eCD185 is also known as CXCR5. It is the receptor for chemokine CXCL13\/BLC, which is chemotactic for B cells.  CXCR5 is expressed on B cells and a subset of T cells in the spleen, neuronal tissue, lymph nodes, and bone marrow.  It is important for migration of B cells into the B cell follicles of the spleen and Peyer’s patches.  Follicular helper T cells (Tfh) also express CXCR5 and the ability of these cells to migrate to the lymph node is modulated by the balanced expression of CCR7 and CXCR5.\u003cbr\u003e\n500microl\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: mCXCR5-transfected cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450\/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: Clone L138D7 staining works optimally at room temperature or 4°C. Unlike other chemokine receptor antibodies, avoid using L138D7 at 37°C.\u003cbr\u003e\nProduct Citations: Wrzner K, et al. 2021. EBioMedicine. 63:103197. PubMed Ludwig J, et al. 2023. NPJ Vaccines. 8:52. PubMed Zhang YN, et al. 2023. Nat Commun. 14:1985. PubMed Jiang W, et al. 2021. Sci Rep. 11:1864. PubMed Chaurio RA, et al. 2022. Immunity. 55:115. PubMed Koenig A, et al. 2022. Front Immunol. 12:791100. PubMed Oishi K, et al. 2022. Cell Rep. 39:111002. PubMed Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed Vanderleyden I, et al. 2020. Cell Rep. 30:611. PubMed Wilfahrt D, et al. 2021. Elife. 10:. PubMed Hill DL, et al. 2021. Elife. 10:. PubMed Webb LMC, et al. 2021. Aging Cell. 20:e13295. PubMed Kim CJ, et al. 2018. Immunity. 49:1034. PubMed Ersching J et al. 2017. Immunity. 46(6):1045-1058 . PubMed Sato R, et al. 2020. Int Immunol. 499:32. PubMed Snell LM, et al. 2018. Immunity. 49:678. PubMed Yue X, et al. 2019. Nat Commun. 10:2011. PubMed Vogel AB, et al. 2021. Nature. 592:283. PubMed Chauveau L, et al. 2021. EMBO Rep. 22:e52447. PubMed Luck H, et al. 2019. Nat Commun. 10:3650. PubMed Zhang YN, et al. 2021. Sci Adv. 7:eabj3107. PubMed Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed Dheilly E, et al. 2020. Cancer Cell. 37(5):674-689. PubMed Lederer K, et al. 2020. Immunity. 53(6):1281-1295.e5. PubMed Smith LK, et al. 2021. Elife. 10:. PubMed Trindade BC, et al. 2021. Immunity. 54:2273. PubMed\u003cbr\u003e\nRRID: AB_2562127 (BioLegend Cat. No. 145511) AB_2562128 (BioLegend Cat. No. 145512)\u003cbr\u003e\nStructure: 42 kD, seven transmembrane protein\u003cbr\u003e\nDistribution: B cells and a subset of T cells in mouse spleen, Tfh cells, also expressed in lymph node, bone marrow, and brain\u003cbr\u003e\nFunction: B cell migration into follicles, possible regulatory role in B cell differentiation and Burkitt's lymphoma\u003cbr\u003e\nLigand\/Receptor: BLC\/CXCL13\u003cbr\u003e\nCell Type: B cells, T cells, Tfh\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors, GPCR\u003cbr\u003e\nAntigen References: 1. Kaiser E, et al. 1993. Eur. J. Immunol. 23:2532. 2. Forster R, et al. 1994. Cell. Mol. Biol. 40:381. 3. Forster R, et al. 1994. Blood 84:830. 4. Forster R, et al. 1996. Cell 87:1037. 5. Wolf I, et al. 1998. J. Biol. Chem. 273:28831.\u003cbr\u003e\nGene ID: 12145\u003cbr\u003e\nUniProt: View information about CD185 on UniProt.org\u003cbr\u003e\nClone: L138D7\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: BLR1, GPCR6, MDR-15\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003cbr\u003e\nQ: What is the F\/P ratio range of our BV421™ format antibody reagents?\u003cbr\u003e\nA: It is lot-specific. On average it ranges between 2-4.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866118508713,"sku":"145512","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-145512","provider":"Iright","version":"1.0","type":"link"}