{"product_id":"biolegend-148707","title":"Biolegend, 148707, PE\/Cyanine7 anti-mouse P2X7R Antibody, 25μg","description":"\u003cp\u003eP2X7R, also known as P2X7 receptor, belongs to the family of ligand-gated ion channel receptors. It is expressed on T cells, B cells, macrophages, and microglia. The receptor opens in the presence of extracellular ATP or NAD, leading to intracellular calcium mobilization. P2X7R activation requires higher concentrations of ATP compared to other P2X receptors. Longer stimulation results in larger pores, allowing passage of larger molecules. Activation of these molecules also leads to mitochondrial and cytoskeletal changes as well as IL-1β maturation and release. Ligation of P2X7 receptor can lead to membrane blebbing and cell death.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Murine colon mast cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE\/Cyanine7 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 1 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications for the relevant formats include: immunoprecipitation1, Western blotting1, immunohistochemistry1, in vivo inhibition of intestinal inflammation and mast cell activation1.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Kurashima Y, et al. 2012. Nat. Commun. 3:1034. (FC, IP, WB, IHC, FA)\u003cbr\u003e\nProduct Citations: Lin YH 2023. Immunity. 56(1):207-223.e8. PubMed Wennerberg E, et al. 2022. Sci Transl Med. 14:eabe8195. PubMed von Roemeling CA, et al. 2020. Nat Commun. 11:1508. PubMed Scheyltjens I, et al. 2022. Nat Protoc. 17:2354. PubMed\u003cbr\u003e\nRRID: AB_2721685 (BioLegend Cat. No. 148707) AB_2721686 (BioLegend Cat. No. 148708)\u003cbr\u003e\nStructure: Ligand-gated ion channel, trimer - two transmembrane regions and one cytosolic region.\u003cbr\u003e\nDistribution: Immune cells, including mast cells, microglia, macrophages, T cells, and B cells.\u003cbr\u003e\nFunction: Ion channel activated by extracellular ATP.\u003cbr\u003e\nLigand\/Receptor: ATP, NAD.\u003cbr\u003e\nCell Type: B cells, Macrophages, Mast cells, T cells, Tregs\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nAntigen References: 1. Surprenant A, et al. 1996. Science. 272:735. 2. Chused TM, et al. 1996. J. Immunol. 157:1371. 3. Gargett CE, et al. 1997. Br. J. Pharmacol. 122:911. 4. Kawamura H, et al. 2006. J. Immunol. 176:2152. 5. Pelegrin P. 2011. Br. J. Pharmacol. 163:908. 6. Bartlett R, et al. 2014. Pharmacol. Rev. 66:638. 7. Alves LA, et al. 2014. Biochim. Biophys. Acta. 1838:2578.\u003cbr\u003e\nGene ID: 18439\u003cbr\u003e\nUniProt: View information about P2X7R on UniProt.org\u003cbr\u003e\nClone: 1F11\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: P2X7 receptor (P2X7R), P2RX7\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860858032297,"sku":"148707","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-148707","provider":"Iright","version":"1.0","type":"link"}