{"product_id":"biolegend-149524","title":"Biolegend, 149524, Alexa Fluor® 488 anti-mouse CD16.2 (FcγRIV) Antibody, 100μg","description":"\u003cp\u003eFcγRIV, also known as CD16.2, is an intermediate-affinity activating receptor for IgG2a and IgG2b. CD16.2 is the mouse homolog of human FcγRIIIA. CD16.2 is a low-affinity IgE receptor for all allotypes and the ligation of FcγRIV by antigen-IgE immune complexes promotes macrophage-mediated phagocytosis and is involved in lung inflammation.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Armenian Hamster\u003cbr\u003e\nImmunogen: FCγR4 —EC domain fusion with IgG1 Fc\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats of this clone) include: blocking of Fc?RIV function1 and inhibition of immune complex binding1,2. The LEAF™ or Ultra-LEAF™ purified antibody (Endotoxin \u0026lt; EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (contact our custom solutions team).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Mancardi DA, et al. 2008. J. Clin. Invest 118:3738. (FC, Block) Nimmerjahn F, et al. 2005. Immunity 23:41.\u003cbr\u003e\nProduct Citations: Liu H, et al. 2022. Cell Rep Med. 3:100660. PubMed\u003cbr\u003e\nRRID: AB_2687087 (BioLegend Cat. No. 149523) AB_2687088 (BioLegend Cat. No. 149524)\u003cbr\u003e\nStructure: 22.4 kD\u003cbr\u003e\nDistribution: Expressed on peripheral blood leukocytes, spleen, colon, bone marrow, and the intestine\u003cbr\u003e\nFunction: Activating receptor for IgG2a and IgG2b via association with an ITAM bearing accessory.\u003cbr\u003e\nInteraction: FCγR4 –EC domain fusion with IgG1 Fc\u003cbr\u003e\nLigand\/Receptor: IgG2a and IgG2b\u003cbr\u003e\nCell Type: B cells, Leukocytes\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, Fc Receptors\u003cbr\u003e\nAntigen References: Mechetina LV, et al. 2002. Immunogenetics. 54:463-8. Nimmerjahn F, et al. 2005. Immunity. 23:41-51. Seeling M, et al. 2013. Proc. Natl. Acad. Sci. 110:10729-34.\u003cbr\u003e\nGene ID: 246256\u003cbr\u003e\nUniProt: View information about CD16.2 on UniProt.org\u003cbr\u003e\nClone: 9E9\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: FcgRIV, Fcgr4, Fcrl3, CD16-2, FcgRIV, Fcgr3a, FcgammaRIV, 4833442P21Rik, Fc gamma RIV\u003cbr\u003e\nIsotype: Armenian Hamster IgG\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862509736105,"sku":"149524","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-149524","provider":"Iright","version":"1.0","type":"link"}