{"product_id":"biolegend-149704","title":"Biolegend, 149704, PE anti-mouse Granzyme A Antibody, 100μg","description":"\u003cp\u003eGranzyme A is a serine protease that is similar to Granzyme B. However, unlike granzyme B, granzyme A is a homodimer. It is expressed in cytotoxic T cells and natural killer cells (NK), but is not essential for induction of apoptosis or DNA fragmentation. Cell death induced by granzyme A is slower and morphologically different than the cell death induced by granzyme B. It plays a crucial role in infection-mediated inflammation as well as immunological responses against viruses, bacteria, and tumor cells.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Full length recombinant granzyme A of mouse origin.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Fehniger TA, et al. 2007. Immunity 26:798. (ICFC)\u003cbr\u003e\nProduct Citations: Corria-Osorio J, et al. 2023. Nat Immunol. 24:869. PubMed Saxena V, et al. 2022. Cell Rep. 39:110727. PubMed Santiago L, et al. 2020. Cell Rep. 107847:32. PubMed Findlay EG, et al. 2019. Oncoimmunology. 8:1608106. PubMed Wu J, et al. 2020. Sci Adv. 6:eaba3458. PubMed Jiang P, et al. 2021. Nat Methods. 18:1181. PubMed Uchil PD et al. 2018. Cell host \u0026amp; microbe. 25(1):87-100 . PubMed Wiedemann GM, et al. 2020. Cell Rep. 33:108498. PubMed Souza COS, et al. 2021. iScience. 24(6):102548. PubMed Thom SR, et al. 2017. J Appl Physiol (1985). 123:297. PubMed\u003cbr\u003e\nRRID: AB_2565309 (BioLegend Cat. No. 149703) AB_2565310 (BioLegend Cat. No. 149704)\u003cbr\u003e\nStructure: 29 kD, Homodimer, Serine protease\u003cbr\u003e\nDistribution: Cytotoxic T cells and natural killer cells (NK); low on granulocytes, B cells, and activated dendritic cells (DCs).\u003cbr\u003e\nFunction: Induction of cell death and apoptosis.\u003cbr\u003e\nCell Type: T cells, NK cells\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience\u003cbr\u003e\nMolecular Family: Proteases, Enzymes and Regulators\u003cbr\u003e\nAntigen References: 1. Susanto O, et al. 2013. Cell Death Differ. 20:1183. 2. Ebnet K, et al. 1995. EMBO J. 14:4230. 3. Jenne DE, et al. 1988. Immunol. Rev. 103:53.\u003cbr\u003e\nGene ID: 14938\u003cbr\u003e\nUniProt: View information about Granzyme A on UniProt.org\u003cbr\u003e\nClone: 3G8.5\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Hf, SE1, TSP1, Ctla3, TSP-1, Ctla-3, AW494114\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862399766697,"sku":"149704","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-149704","provider":"Iright","version":"1.0","type":"link"}