{"product_id":"biolegend-150004","title":"Biolegend, 150004, Alexa Fluor® 647 anti-mouse Blimp-1 Antibody, 100μg","description":"\u003cp\u003eBlimp-1, also known as PRDM1, is a 98 kD protein containing 5 Kruppel-type zinc finger domains. Blimp-1 represses the transcription factors BCL6 and c-Myc. It is the master regulator of terminal B cell differentiation and is also involved in the differentiation and homeostasis of T cells and natural killer (NK) cells.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Amino acids 255-395 from mouse Blimp-1 fused with GST.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality testedWB - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm \/ 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.\u003cbr\u003e\nProduct Citations: Chen HY, et al. 2022. Elife. 11:. PubMed Roco JA et al. 2019. Immunity. 51(2):337-350 . PubMed Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed Piper CJM, et al. 2020. Cell Reports. 29(7):1878-1892.e7.. PubMed Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed Snell LM, et al. 2018. Immunity. 49:678. PubMed Venturutti L, et al. 2020. Cell. 182(2):297-316.e27. PubMed Roy K, et al. 2019. Immunity. 50:616. PubMed Zhu H, et al. 2019. Nat Commun. 10:1084. PubMed Cho S, et al. 2018. Nat Commun. 9:2757. PubMed Sanchez HN, et al. 2020. Nat Commun. 0.5. PubMed Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed Kunishita Y, et al. 2020. Front Immunol. 11:98. PubMed\u003cbr\u003e\nRRID: AB_2565617 (BioLegend Cat. No. 150003) AB_2565618 (BioLegend Cat. No. 150004)\u003cbr\u003e\nStructure: Five Kruppel-type zinc finger domains, 98 kD.\u003cbr\u003e\nDistribution: Plasma cells, CD4+ and CD8+ effector\/memory T cells, and natural killer cells.\u003cbr\u003e\nFunction: Terminal differentiation of B cells, effector\/memory T cells, role in natural killer cells (NK) and T cell homeostasis, and repressor of BCL6 and c-Myc.\u003cbr\u003e\nCell Type: Plasma cells, T cells, NK cells, B cells\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Transcription Factors\u003cbr\u003e\nAntigen References: 1. Nakaki F, et al. 2013. Nature 501:222. 2. Crotty S, et al. 2010. Nat. Immunol. 11:114. 3. Zhao WL, et al. 2008. Blood 111:3867. 4. Climmino L, et al. 2008. J. Immunol. 181:2338. 5. Martins, G and Clarne, K. 2008. Annu. Rev. Immunol. 26:133.\u003cbr\u003e\nGene ID: 12142\u003cbr\u003e\nUniProt: View information about Blimp-1 on UniProt.org\u003cbr\u003e\nClone: 5E7\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: PR domain zinc finger protein 1, B lymphocyte-induced maturation protein-1, PRDM1, BLIMP1, PRDI-BF1, ZNFPR1A1, PRDM-1, BLIMP1\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864147120297,"sku":"150004","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-150004","provider":"Iright","version":"1.0","type":"link"}