{"product_id":"biolegend-151204","title":"Biolegend, 151204, Alexa Fluor® 488 anti-mouse\/human Ki-67 Antibody, 100μg","description":"\u003cp\u003eThe nuclear protein Ki-67 was first identified by the monoclonal antibody Ki-67, which was generated by immunizing mice with nuclei of the L428 Hodgkin lymphoma cell line. Ki-67 protein plays an essential role in ribosomal RNA transcription and cell proliferation. Expression of Ki-67 occurs during G1, S, G2, and M phase. While in G0 phase, the Ki-67 protein is not detectable. Ki-67 is strongly expressed in proliferating cells and has been reported as a prognostic marker in various tumors.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse, Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: E. coli expressed, N-terminal His-Thioredoxin-tagged, partial mKi-67 (1816-2163 aa) recombinant protein.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested ICC, IHC-F - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For intracellular flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg\/ml is recommended. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 1.25 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nProduct Citations: Chan S, et al. 2022. Nat Cancer. . PubMed Kuczynski EA, et al. 2022. EMBO Mol Med. 14:e15816. PubMed Wei Z, et al. 2021. Invest Ophthalmol Vis Sci. 62:29. PubMed Ni F, et al. 2019. Cell Stem Cell. 24:608. PubMed Huang FL, et al. 2022. Oncol Rep. 47:. PubMed Gao H, et al. 2021. Cells. 10:. PubMed Yi W, et al. 2021. Cell Reports. 34(13):108922. PubMed Lu J, et al. 2021. Cell Death Discov. 7:165. PubMed\u003cbr\u003e\nRRID: AB_2566800 (BioLegend Cat. No. 151204)\u003cbr\u003e\nStructure: 325 kD protein containing a forkhead-associated (FHA) domain and 13 tandem repeats.\u003cbr\u003e\nDistribution: Nucleus and chromosomes.\u003cbr\u003e\nFunction: Required for cell cycle progression and proliferation.\u003cbr\u003e\nBiology Area: Cell Biology, Cell Cycle\/DNA Replication\u003cbr\u003e\nAntigen References: Starborg M, et al. 1996. J. Cell. Sci. 109:143. Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987. Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174. Beltrami AP, et al. 2001. N. Engl. J. Med. 344:1750. Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295. Nagy Z, et al. 1997. Acta. Neuropathol. 93:294.\u003cbr\u003e\nGene ID: 428817345\u003cbr\u003e\nUniProt: View information about Ki-67 on UniProt.org\u003cbr\u003e\nClone: 11F6\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Mki67, Ki67, Ki-67, MIB-1, KIA\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864326033577,"sku":"151204","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-151204","provider":"Iright","version":"1.0","type":"link"}