{"product_id":"biolegend-154202","title":"Biolegend, 154202, Purified anti-mouse CD25 Antibody, 500μg","description":"\u003cp\u003eCD25, also known as IL-2 low affinity receptor is a type I, 55 kDa, transmembrane glycoprotein. CD25 It is expressed on activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132 (common γ chain), CD25 forms the high affinity signaling IL-2 receptor.\u003cbr\u003e\n500μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: HT-2 cell line\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality testedBlock, Depletion, IP - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: Inhibition of IL-2 dependent proliferation of HT2 cells, immunoprecipitation of CD25 from cell lysates, in vivo depletion of CD25+ cells.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Malek TR, et al. 1983. Proc. Natl. Acad. Sci. USA. 80:5694. (FC, Block, IP) Couper KN. 2007. J. Immunol. 178:4136. (Depletion)\u003cbr\u003e\nProduct Citations: Jayachandran R, et al. 2019. Immunity. 50:152. PubMed Zhou D, et al. 2022. Nat Commun. 13:5174. PubMed\u003cbr\u003e\nRRID: AB_2716173 (BioLegend Cat. No. 154202)\u003cbr\u003e\nStructure: Type I, 55 kD, transmembrane glycoprotein\u003cbr\u003e\nDistribution: Activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells\u003cbr\u003e\nLigand\/Receptor: IL-2\u003cbr\u003e\nCell Type: B cells, T cells, Thymocytes, Tregs\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors\u003cbr\u003e\nAntigen References: 1. Huss DJ, et al. 2015. J. Immunol. 194:84. 2. Hartmann FJ, et al. 2014. Nat. Commun. 5:5056. 3. Codarri L, et al. 2007. J. Exp. Med. 204:1533. 4. Read S, et al. 2000. J. Exp. Med. 192:295. 5. Taniguchi T, et al. 1993. Cell 73:5.\u003cbr\u003e\nGene ID: 3559\u003cbr\u003e\nUniProt: View information about CD25 on UniProt.org\u003cbr\u003e\nClone: 7D4\/CD25\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: IL-2Rα, IL2RA, IL-2Ra, Ly-43, p55, Tac\u003cbr\u003e\nIsotype: Rat IgM, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862487126185,"sku":"154202","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-154202","provider":"Iright","version":"1.0","type":"link"}