{"product_id":"biolegend-162321","title":"Biolegend, 162321, Brilliant Violet 750™ anti-mouse CD8a Antibody, 50μg","description":"\u003cp\u003eCD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)\/β(CD8b) heterodimer and α\/α homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 α\/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α\/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α\/α homodimer is expressed on a subset of γ\/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Recombinant mouse CD8a\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 750™ under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 750™ excites at 405 nm and emits at 750 nm. The bandpass filter 780\/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 750™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nRRID: AB_3698924 (BioLegend Cat. No. 162321)\u003cbr\u003e\nStructure: Ig superfamily, CD8α chain, 34 kD\u003cbr\u003e\nDistribution: Most thymocytes, T cell subset, some NK cells, lymphoid dendritic cells\u003cbr\u003e\nFunction: Co-receptor for TCR\u003cbr\u003e\nLigand\/Receptor: MHC class I molecule\u003cbr\u003e\nCell Type: Dendritic cells, NK cells, T cells, Thymocytes, Tregs\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IHC, IP) 2. Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1. (Deplete) 3. Takahashi K, et al. 1992. P. Natl. Acad. Sci. USA 89:5557. (Block, IP) 4. Ledbetter JA, et al. 1981. J. Exp. Med. 153:1503. (Block) 5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC) 6. Fan WY, et al. 2001. Exp. Biol. Med. 226:1045. (IHC) 7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC) 8. Kamimura D, et al. 2006. J. Immunol. 177:306. 9. Bouwer HGA, et al. 2006. P. Natl. Acad. Sci. USA 103:5102. (FC, Deplete) 10. Kao C, et al. 2005. Int. Immunol. 17:1607. PubMed 11. Ko SY, et al. 2005. J. Immunol. 175:3309. (FC) PubMed 12. Rasmussen JW, et al. 2006. Infect. Immun. 74:6590\u003cbr\u003e\nGene ID: 12525\u003cbr\u003e\nUniProt: View information about CD8alpha on UniProt.org\u003cbr\u003e\nClone: S18018E\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: T8, Lyt2, Ly-2\u003cbr\u003e\nIsotype: Rat IgG2b, λ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866252464297,"sku":"162321","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-162321","provider":"Iright","version":"1.0","type":"link"}