{"product_id":"biolegend-163904","title":"Biolegend, 163904, Ultra-LEAF™ Purified anti-mouse CD182 (CXCR2) Antibody, 1mg","description":"\u003cp\u003eCD182, also known as CXCR2, is a G-protein coupled receptor with 7 transmembrane regions that is involved in chemotaxis, neutrophil activation and angiogenesis. CXCR2 is expressed by neutrophils, basophils, subset of T cells, monocytes, macrophages, NK and NKT cells. Its ligands are CXCL1, CXCL2, CXCL3 and CXCL5.\u003cbr\u003e\n1mg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Pre-B cell transfected with mCXCR2\u003cbr\u003e\nFormulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.\u003cbr\u003e\nEndotoxin Level: Less than 0.01 EU\/µg of the protein (\u0026lt; 0.001 ng\/µg of the protein) as determined by the LAL test.\u003cbr\u003e\nPreparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg\/mL and 3 mg\/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.\u003cbr\u003e\nApplication: FC - Quality tested Block - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Clone 3F10-B3 blocks the chemotaxis towards CXCR2 ligands.\u003cbr\u003e\nRRID: AB_2927843 (BioLegend Cat. No. 163903) AB_2927843 (BioLegend Cat. No. 163904)\u003cbr\u003e\nStructure: G-protein coupled receptor, 7 transmembrane regions.\u003cbr\u003e\nDistribution: Neutrophils, basophils, subset of T cells, monocytes, macrophages, and natural killer cells (NK)\u003cbr\u003e\nFunction: Chemotaxis, neutrophil activation, and angiogenesis\u003cbr\u003e\nLigand\/Receptor: CXCL1, CXCL2, CXCL3, and CXCL5\u003cbr\u003e\nCell Type: Neutrophils\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors, GPCR\u003cbr\u003e\nAntigen References: Spaan AN, et al. 2014. Nat Commun. 5:5438. Natsuaki Y, et al. 2014. Nat. Immunol. 11:1064-9. Hsieh CY, et al. 2014. J Immunol. 193:3693-703.\u003cbr\u003e\nGene ID: 12765\u003cbr\u003e\nUniProt: View information about CD182 on UniProt.org\u003cbr\u003e\nClone: 3F10-B3\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: IL-8RB, GRO\/MGSA, Cmkar2\u003cbr\u003e\nIsotype: Mouse IgG2c, κ\u003cbr\u003e\nQ: Do you guarantee that your antibodies are totally pathogen free?\u003cbr\u003e\nA: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.\u003cbr\u003e\nQ: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?\u003cbr\u003e\nA: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.\u003cbr\u003e\nQ: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?\u003cbr\u003e\nA: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.\u003cbr\u003e\nQ: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?\u003cbr\u003e\nA: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862395375785,"sku":"163904","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-163904","provider":"Iright","version":"1.0","type":"link"}