{"product_id":"biolegend-200306","title":"Biolegend, 200306, FITC anti-rat CD86 Antibody, 500μg","description":"\u003cp\u003eCD86 is an 80 kD member of the immunoglobulin superfamily also known as B70 or B7-2. It is expressed on antigen presenting cells and activated T and B cells. CD86 is a ligand for CD28 and CD152 (CTLA-4). It is one of the accessory molecules that plays an important role in T cell-APC cell costimulatory interactions. The 24F antibody can block the co-stimulatory activity of CD86.\u003cbr\u003e\n500μg\u003cbr\u003e\nVerified Reactivity: Rat\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: HTLV-1-transformed Lewis-S1 rat T cell line\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, blocking of costimulation of T cells1-3, and immunohistochemical staining of acetone-fixed frozen sections3. The LEAF™ purified antibody (Endotoxin \u0026lt;0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 200310).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Maeda K, et al. 1997. Int. Immunol. 9:993. (IP, Block)Bluestone JA. 1995. Immunity 2:555. (IP, Block)Damoiseaux JG, et al. 1998. J. Leuk. Biol. 64:803. (IP, Block, IHC)Donius LR, et al. 2014. Immunobiology. 219:440. PubMed\u003cbr\u003e\nProduct Citations: Li X, et al. 2013. Mol Cell Neurosci. 40:7431. PubMed Li B, et al. 2020. Cell Mol Immunol. 1.386111111. PubMed Hu S, et al. 2008. Mol Immunol. 45:3818. PubMed\u003cbr\u003e\nRRID: AB_313854 (BioLegend Cat. No. 200305)\u003cbr\u003e\nStructure: Ig superfamily, 80 kD\u003cbr\u003e\nDistribution: Antigen presenting cells, activated B cells and T cells\u003cbr\u003e\nFunction: T and B cell costimulation\u003cbr\u003e\nLigand\/Receptor: CD28, CD152 (CTLA-4)\u003cbr\u003e\nCell Type: B cells, T cells, Antigen-presenting cells\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Costimulatory Molecules\u003cbr\u003e\nMolecular Family: CD Molecules, Immune Checkpoint Receptors\u003cbr\u003e\nAntigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook. Academic Press. 2. Hathcock KS, et al. 1993. Science 262:905. 3. Freeman GJ, et al. 1993. Science 262:907. 4. Carreno BM, et al. 2002. Ann. Rev. Immunol. 20:29.\u003cbr\u003e\nGene ID: 56822\u003cbr\u003e\nUniProt: View information about CD86 on UniProt.org\u003cbr\u003e\nClone: 24F\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: B7-2, B70\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860876382377,"sku":"200306","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-200306","provider":"Iright","version":"1.0","type":"link"}