{"product_id":"biolegend-200308","title":"Biolegend, 200308, PE anti-rat CD86 Antibody, 200μg","description":"\u003cp\u003eCD86 is an 80 kD member of the immunoglobulin superfamily also known as B70 or B7-2. It is expressed on antigen presenting cells and activated T and B cells. CD86 is a ligand for CD28 and CD152 (CTLA-4). It is one of the accessory molecules that plays an important role in T cell-APC cell costimulatory interactions. The 24F antibody can block the co-stimulatory activity of CD86.\u003cbr\u003e\n200μg\u003cbr\u003e\nVerified Reactivity: Rat\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: HTLV-1-transformed Lewis-S1 rat T cell line\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, blocking of costimulation of T cells1-3, and immunohistochemical staining of acetone-fixed frozen sections3. The LEAF™ purified antibody (Endotoxin \u0026lt;0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 200310).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Maeda K, et al. 1997. Int. Immunol. 9:993. (IP, Block)Bluestone JA. 1995. Immunity 2:555. (IP, Block)Damoiseaux JG, et al. 1998. J. Leuk. Biol. 64:803. (IP, Block, IHC)Donius LR, et al. 2014. Immunobiology. 219:440. PubMed\u003cbr\u003e\nProduct Citations: Zhong S, et al. 2023. J Ultrasound Med. 42:463. PubMed Ren J, et al. 2023. Cell Death Dis. 14:70. PubMed Gu G, et al. 2023. Cell Biosci. 13:23. PubMed Zonneveld-Huijssoon E, et al. 2012. Ann Rheum Dis. 71:1706. PubMed Li J, et al. 2022. Am J Transl Res. 14:5003. PubMed Fu Y, et al. 2020. Mol Ther. 1214:28. PubMed Wu H, et al. 2016. Sci Rep. 6: 24548. PubMed Martín-Fernández B, et al. 2016. PLoS One. 11: 0145946. PubMed Snell LM, et al. 2018. Immunity. 49:678. PubMed Li Y, et al. 2020. Front Immunol. 1.577083333. PubMed Lin ZH, et al. 2018. Front Cell Neurosci. 0.729861111. PubMed Wang N, et al. 2018. Exp Ther Med. 15:1906. PubMed Xin L, et al. 2022. Adv Healthc Mater. 11:e2201680. PubMed Treacy O, et al. 2012. PLoS One. 7:e42662. PubMed\u003cbr\u003e\nRRID: AB_313856 (BioLegend Cat. No. 200307) AB_313857 (BioLegend Cat. No. 200308)\u003cbr\u003e\nStructure: Ig superfamily, 80 kD\u003cbr\u003e\nDistribution: Antigen presenting cells, activated B cells and T cells\u003cbr\u003e\nFunction: T and B cell costimulation\u003cbr\u003e\nLigand\/Receptor: CD28, CD152 (CTLA-4)\u003cbr\u003e\nCell Type: B cells, T cells, Antigen-presenting cells\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Costimulatory Molecules\u003cbr\u003e\nMolecular Family: CD Molecules, Immune Checkpoint Receptors\u003cbr\u003e\nAntigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook. Academic Press. 2. Hathcock KS, et al. 1993. Science 262:905. 3. Freeman GJ, et al. 1993. Science 262:907. 4. Carreno BM, et al. 2002. Ann. Rev. Immunol. 20:29.\u003cbr\u003e\nGene ID: 56822\u003cbr\u003e\nUniProt: View information about CD86 on UniProt.org\u003cbr\u003e\nClone: 24F\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: B7-2, B70\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860876644521,"sku":"200308","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-200308","provider":"Iright","version":"1.0","type":"link"}