{"product_id":"biolegend-200702","title":"Biolegend, 200702, Purified anti-rat CD8b Antibody, 500μg","description":"\u003cp\u003eCD8b antigen is also known as T8, Lyt2, Ly-2, and CD8β. It is a member of the immunoglobulin superfamily expressed on most thymocytes, some mature T cell subsets, and macrophages. CD8b forms heterodimers with the CD8α chain (CD8a). CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 participates in T cell activation through its association with the T cell receptor complex and protein tyrosine kinase lck (p56lck).\u003cbr\u003e\n500μg\u003cbr\u003e\nVerified Reactivity: Rat\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: CD8+ Wistar rat splenic T cell hybridomas\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality testedIP, WB, IHC-F - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, immunohistochemical staining1,2 of acetone-fixed frozen sections and Zinc-fixed paraffin-embeded sections, in vitro blocking of allogeneic responses1, Macrophage stimulation3,4 and in vivo depletion.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Torres-Nagel N, et al. 1992. Eur. J. Immunol. 22:2841. (IP IHC WB) Kuhnlein P, et al. 1994. J. Immunol. 153:979. (IHC) Hirji N, et al. 1997. J. Immunol. 158:1833. Hirji N, et al. 1998. J. Immunol. 160:6004.\u003cbr\u003e\nProduct Citations: Tang-Huau TL, et al. 2021. Viruses. 13: . PubMed\u003cbr\u003e\nRRID: AB_313883 (BioLegend Cat. No. 200702)\u003cbr\u003e\nStructure: Ig superfamily, CD8β chain\u003cbr\u003e\nDistribution: Most thymocytes, subset of mature T cells (i.e. including most T suppressor\/cytotoxic cells), macrophages\u003cbr\u003e\nFunction: Co-receptor for TCR\u003cbr\u003e\nLigand\/Receptor: MHC class I molecule\u003cbr\u003e\nCell Type: Macrophages, T cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Torres-Nagel N, et al. 1992. Eur. J. Immunol. 22:2841. 2. Kuhnlein P, et al. 1994. J. Immunol. 153:979. 3. Hirji N, et al. 1997. J. Immunol. 158:1833. 4. Hirji N, et al. 1998. J. Immunol. 160:6004.\u003cbr\u003e\nGene ID: 24931\u003cbr\u003e\nUniProt: View information about CD8beta on UniProt.org\u003cbr\u003e\nClone: 341\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: CD8, Lyt3\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862376566953,"sku":"200702","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-200702","provider":"Iright","version":"1.0","type":"link"}