{"product_id":"biolegend-201905","title":"Biolegend, 201905, APC anti-rat CD11a Antibody, 100μg","description":"\u003cp\u003eCD11a is a 160-170 kD glycoprotein also known as α L integrin, or LFA-1 α chain. It is a member of the integrin family and forms a heterodimer with β 2 integrin (CD18). The LFA-1 (CD11a\/CD18) is broadly expressed on leukocytes and mediates intercellular adhesion and cellular activation. CD54 (ICAM-1), CD102 (ICAM-2), and CD50 (ICAM-3) are the ligands of LFA-1.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Rat\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: PHA-stimulated rat splenocytes and rat thymic lymphoma FTL-43\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: The WT.1 antibody recognizes both activated and unactivated LFA-1 and inhibits the binding of LFA-1 to ICAM-1. The WT.1 antibody has been shown to inhibit the mixed lymphocyte reaction, leukocyte infiltration, and graft rejection. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections1,2, immunoprecipitation3, in vivo and in vitro blocking of cellular adhesion3-6.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Andrews FJ, et al. 1997. Dig. Dis. Sci. 42:326. (IHC) Springer TA. 1994. Cell 76:301. (IHC) Tamatani T, et al. 1991. Eur. J. Immunol. 21:627. (IP, Block) Devine L, et al. 1996. Immunol. 88:456. (Block) Ohta Y, et al. 1998. Transplantation 65:1094. (Block) Watanabe T, et al. 1997. Am. J. Pathol. 150:971. (Block)\u003cbr\u003e\nRRID: AB_2888909 (BioLegend Cat. No. 201904) AB_2888909 (BioLegend Cat. No. 201905)\u003cbr\u003e\nStructure: 160-170 kD glycoprotein\u003cbr\u003e\nDistribution: Leukocytes including lymphocytes, monocytes\/macrophages, and granulocytes.\u003cbr\u003e\nFunction: Mediates intercellular adhesion and cellular activation.\u003cbr\u003e\nInteraction: CD18\u003cbr\u003e\nLigand\/Receptor: CD54 (ICAM-1), CD102 (ICAM-2), CD50 (ICAM-3)\u003cbr\u003e\nBiology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Tamatani T, et al. 1991. Eur. J. Immunol. 21:627. 2. Tamatani T, et al. 1991. Eur. J. Immunol. 21:855.\u003cbr\u003e\nGene ID: 308995\u003cbr\u003e\nUniProt: View information about CD11a on UniProt.org\u003cbr\u003e\nClone: WT.1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: LFA-1 α chain, αL Integrin, Lymphocyte function-associated antigen 1, LFA-1 α subunit, ITGAL, Ly-15, Ly-21\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864097542313,"sku":"201905","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-201905","provider":"Iright","version":"1.0","type":"link"}