{"product_id":"biolegend-205802","title":"Biolegend, 205802, Purified anti-rat CD1d Antibody, 100μg","description":"\u003cp\u003eCD1d is a type I transmembrane protein and member of the MHC family that associates with β2-microglobulin on the cell surface. CD1d is expressed by antigen presenting cells (such as dendritic cells, monocytes, macrophages and B cells), thymocytes, and intestinal epithelial cells. CD1d presents glycolipids to Vα14 i TCR + i NKT cells.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Rat, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: M12.4.1.C3 cells transduced with rat CD1d\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested IHC-F, IP, WB - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: blocking of CD1d-mediated antigen presentation to type I and II NKT cells1, immunoprecipitation1, Western Blotting1, and immunohistochemical staining of frozen tissue sections1. Clones WTH-1 and WTH-2 recognize different epitopes of CD1d.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Monzon-Casanova E, et al. 2010. PLoS ONE 5:e13089.\u003cbr\u003e\nRRID: AB_11147755 (BioLegend Cat. No. 205802)\u003cbr\u003e\nStructure: Member of the MHC family, type I transmembrane protein, associates with β2-microglobulin\u003cbr\u003e\nDistribution: Dendritic cells, monocytes, macrophages, B cells, T cells, thymocytes, intestinal epithelial cells\u003cbr\u003e\nFunction: Presents glycolipid antigens to iNKT cells\u003cbr\u003e\nLigand\/Receptor: Vα14i TCR\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Epithelial cells, Macrophages, Monocytes, T cells\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: CD Molecules, MHC Antigens, TCRs\u003cbr\u003e\nAntigen References: 1. Arrenberg P, et al. 2010. P. Natl. Acad. Sci. USA. 107:10984. 2. Mattarollo SR, et al. 2010. J. Immunol. 184:5663. 3. Wang J, et al. 2010. P. Natl. Acad. Sci. USA. 107:1535. 4. Nieuwenhuis EE, et al. 2009. J. Clin. Invest. 119:1241.\u003cbr\u003e\nGene ID: 25109\u003cbr\u003e\nUniProt: View information about CD1d on UniProt.org\u003cbr\u003e\nClone: WTH-1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Antigen-presenting glycoprotein CD1d, Cd1d1\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864097706153,"sku":"205802","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-205802","provider":"Iright","version":"1.0","type":"link"}