{"product_id":"biolegend-250204","title":"Biolegend, 250204, PE anti-marmoset CD45 Antibody, 100μg","description":"\u003cp\u003eCD45 is a 180-240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Common Marmoset\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Marmoset mononuclear cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen spleen sections1 and Western blotting1. The 6C9 antibody has been reported to not cross-react with humans, cynomolgus monkeys, or squirrel monkeys.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Ito R, et al. 2008. Immunol. Lett. 121:116. (IHC, WB)\u003cbr\u003e\nProduct Citations: Gordeychuk IV, et al. 2019. Acta Naturae. 10:63. PubMed Ireland RE, et al. 2022. Viruses. 14:. PubMed Geirsdottir L, et al. 2020. Cell. 179(7):1609-1622.e16.. PubMed\u003cbr\u003e\nRRID: AB_2562120 (BioLegend Cat. No. 250204)\u003cbr\u003e\nStructure: Tyrosine phosphatases, type I transmembrane protein\u003cbr\u003e\nDistribution: Hematopoietic cells, except circulating erythrocytes or platelets\u003cbr\u003e\nFunction: TCR and BCR mediated activation\u003cbr\u003e\nLigand\/Receptor: Galectin-1, CD2, CD3, CD4\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Thomas M. 1989. Annu. Rev. Immunol. 7:339. 2. Trowbridge I and Thomas M. 1994. Annu. Rev. Immunol. 12:85.\u003cbr\u003e\nGene ID: 100385061\u003cbr\u003e\nUniProt: View information about CD45 on UniProt.org\u003cbr\u003e\nClone: 6C9\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: LCA, T200\u003cbr\u003e\nIsotype: Mouse IgG1\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862474641577,"sku":"250204","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-250204","provider":"Iright","version":"1.0","type":"link"}