{"product_id":"biolegend-260100","title":"Biolegend, 260100, GMP FITC anti-Cytochrome c Antibody, 100tests","description":"\u003cp\u003eCytochrome c is a 15 kD protein found in the mitochondrial intermembrane space with a heme-binding domain. Cytochrome c is a component of the electron transport chain; the heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Cytochrome c initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9. Cytochrome c interacts with the cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, and Caspase 9 proteins. The 6H2.B4 monoclonal antibody recognizes human, mouse, and rat cytochrome-c and has been shown to be useful for intracellular flow cytometric staining, Western blotting, immunoprecipitation, and immunofluorescence staining.\u003cbr\u003e\n100tests\u003cbr\u003e\nReactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Rat cyt c-OVA\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide, 0.2% (w\/v) BSA (origin USA) and a stabilizer.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.\u003cbr\u003e\nConcentration: 200 µg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: intracellular flow cytometry5, immunofluorescence microscopy3,5, immunoprecipitation4, and immunocytochemistry5.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Goshorn SC, et al. 1991. J. Biol. Chem. 266:2134. Jemmerson R, et al. 1991. Eur. J. Immunol. 21:143. Chandra D, et al. 2002. J. Biol. Chem. 277:50842. (IF) Semenkova L, et al. 2003. Eur. J. Biochem. 270:4388. (IP) Shih S-F, et al. 2001. J. Biol. Chem. 276:21870. (ICFC ICC IF) She P, et al. 2011. Am J. Physiol Endcorinol Metab.301:E49. PubMed McGuire, KA., et al. 2011. J. Virol 85:10806. PubMed\u003cbr\u003e\nDisclaimer: GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include: Batch-to-batch consistency Material traceability Documented procedures Documented employee training Equipment maintenance and monitoring records Lot-specific certificates of analysis Quality audits per ISO 13485:2016 QA review of released products\u003cbr\u003e\nStructure: Heme binding domain; 15 kD\u003cbr\u003e\nDistribution: Mitochondrial intermembrane space\u003cbr\u003e\nFunction: Component of electron transport chain; heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9\u003cbr\u003e\nInteraction: Cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, Casp9\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Mitochondrial Function, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: Mitochondrial Markers\u003cbr\u003e\nAntigen References: Liu X, et al. 1996. Cell. 86:147. Li P, et al. 1997. Cell. 91:479. Zhang Z, et al. 2003. Gene 312:61. Ferguson H, et al. 2003. J. Biol. Chem. 278:45793.\u003cbr\u003e\nGene ID: 1355\u003cbr\u003e\nUniProt: View information about Cytochrome c on UniProt.org\u003cbr\u003e\nClone: 6H2.B4\u003cbr\u003e\nOther Names: Cyt c\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866515951785,"sku":"260100","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-260100","provider":"Iright","version":"1.0","type":"link"}