{"product_id":"biolegend-260322","title":"Biolegend, 260322, Purified anti-Mcl-1 Antibody, 100tests","description":"\u003cp\u003eMcl-1 is a member of the pro-survival BCL-2 family of apoptotic regulatory proteins. Mcl-1 plays a crucial role in the survival and development of various cell types by binding to proapoptotic proteins and inhibiting the mitochondrial apoptotic pathway. Unlike Bcl-2 and Bcl-xL, Mcl-1 lacks a BH4 domain and contains an N-terminal PEST region with specific sequences for ubiquitination, phosphorylation, and caspase cleavage sites, which are important in regulating the half-life. Phosphorylation of at T92 and T163 by ERK inhibits Mcl-1 turnover, whereas phosphorylation by GSK3β at S159 facilitates proteosomal degradation. Increased expression of Mcl-1 is correlated with tumor survival and is associated with resistance of chemotherapeutics.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Recombinant protein comprising a portion of the N terminus of human Mcl-1\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg\/mL. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg\/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nAdditional Product Notes: For use in immunocytochemistry (ICC), it is recommended to fix\/permeabilize with any of the following: -Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol -100% ice-cold methanol only Fixation\/permeabilization with Fixation buffer followed by 0.5 % Triton X-100 is not recommended due to dim signal.\u003cbr\u003e\nRRID: AB_3720577 (BioLegend Cat. No. 648852) AB_3720577 (BioLegend Cat. No. 648853)\u003cbr\u003e\nStructure: Mcl-1 is a 350 amino acid protein with a predicted molecular weight of 37.3 kD.\u003cbr\u003e\nDistribution: Tissue distribution: widely expressed Cellular distribution: primarily mitochondria (outer membrane), also nucleus, cytosol and endoplasmic reticulum\u003cbr\u003e\nFunction: Anti-apoptotic\/pro-survival protein\u003cbr\u003e\nInteraction: Mcl-1 forms a homodimer. Interacts with HIF3A, PMAIP1, BBC3, BAX, BAK1, TPT1, BOP, BCL2L11, BAD, BOK, BIK, and BMF.\u003cbr\u003e\nCell Type: Tregs\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Immunology\u003cbr\u003e\nAntigen References: Kozopas K, et al. 1993. P. Natl. Acad. Sci. USA. 90:3516. Zhou P, et al. 1997. Blood. 89:630. Chao J, et al. 1998. Mol. Cell. Biol. 18:4883. Epling-Burnette P, et al. 2001. J. Immunol. 166:7486. Moulding D, et al. 1998. Blood. 92:2495. Inoshita S, et al. 2002. J. Biol. Chem. 277(46):43740. Opferman JT, et al. 2003. Nature. 426:671.\u003cbr\u003e\nGene ID: 4170\u003cbr\u003e\nUniProt: View information about Mcl-1 on UniProt.org\u003cbr\u003e\nClone: W23229E\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Induced myeloid leukemia cell differentiation protein Mcl-1, Bcl-2-like protein 3 (BCL2-L-3), Bcl-2-related protein EAT\/mcl1\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866439504041,"sku":"260322","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-260322","provider":"Iright","version":"1.0","type":"link"}