{"product_id":"biolegend-260384","title":"Biolegend, 260384, GMP PE anti-human CD95 (Fas) Antibody, 100tests","description":"\u003cp\u003eCD95 is a 45 kD single chain type I glycoprotein also known as Fas, APO-1, and TNFRSF6. It is a member of the TNF receptor superfamily. CD95 is expressed on T and B lymphocytes, monocytes, neutrophils, and fibroblasts. CD95 expression is upregulated by activation. The extracellular region of CD95 binds to CD178 (Fas ligand). CD178 binding to CD95 induces apoptosis and has been shown to play a role in the maintenance of peripheral tolerance.\u003cbr\u003e\n100tests\u003cbr\u003e\nReactivity: Human,Cynomolgus,Rhesus\u003cbr\u003e\nReported Reactivity: African Green, Baboon, Capuchin Monkey, Chimpanzee, Common Marmoset, Cotton-topped Tamarin, Pigtailed Macaque, Sooty Mangabey\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: CD95 transfected L cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w\/v) BSA (origin USA) and a stabilizer.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 100 µg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: The DX2 antibody is useful for inducing apoptosis of Fas-positive cells. Additional reported applications (for the relevant formats) include: in vitro induction of apoptosis3 (DX2 antibody is required to be cross-linked for effective induction of apoptosis) and immunohistochemical staining4,5 of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections. The Ultra-LEAF™ purified antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 305655 and 305656). Note: EOS9.1 antibody can induce apoptosis without cross-linking.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds.1995. Leucocyte Typing V. Oxford University Press. New York. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. New York. Cifone M, et al. 1994. J. Exp. Med. 180:1547. (Apop) Zietz C, et al. 2001. Am. J. Pathol. 159:963. (IHC) Sergi C, et al. 2000. Am. J. Pathol. 156:1589. (IHC) Xie S, et al. 2010. J. Immunol. 184:2289. (FC) PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21. Rout N, et al. 2010. PLoS One 5:e9787. (FC) Dixit N, et al. 2012. J. Immunol. 189:5954. PubMed\u003cbr\u003e\nDisclaimer: GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include: Batch-to-batch consistency Material traceability Documented procedures Documented employee training Equipment maintenance and monitoring records Lot-specific certificates of analysis Quality audits per ISO 13485:2016 QA review of released products\u003cbr\u003e\nStructure: TNFR superfamily, type I transmembrane protein, 45 kD\u003cbr\u003e\nDistribution: T cells and B cells, monocytes, neutrophils, fibroblasts, expression level upregulated on activated lymphocytes\u003cbr\u003e\nFunction: Mediates apoptosis\u003cbr\u003e\nLigand\/Receptor: Fas ligand (CD178)\u003cbr\u003e\nCell Type: B cells, Fibroblasts, Lymphocytes, Monocytes, Neutrophils, T cells\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Immunology, Neuroscience\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Krammer P, et al. 1994. Immunol. Rev. 142:175. 2. Nagata S, et al. 1995. Science 267:1449.\u003cbr\u003e\nGene ID: 355\u003cbr\u003e\nUniProt: View information about CD95 on UniProt.org\u003cbr\u003e\nClone: DX2\u003cbr\u003e\nWorkshop: VI C-64\u003cbr\u003e\nOther Names: Fas, APO-1, TNFRSF6\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866522407081,"sku":"260384","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-260384","provider":"Iright","version":"1.0","type":"link"}