{"product_id":"biolegend-260390","title":"Biolegend, 260390, GMP PerCP\/Cyanine5.5 anti-human CD22 Antibody, 100tests","description":"\u003cp\u003eCD22 is a 130 kD type I transmembrane glycoprotein also known as Siglec-2 and BL-CAM. It is a member of the immunoglobulin superfamily (sialoadhesion subgroup). CD22 is expressed in the cytoplasm of pro-B and pre-B cells, and on the surface of mature B and activated B cells, but not on plasma cells. CD22 is present in the B cell receptor complex and associates with SHP-1, Syk, Lck, Lyn, and phospholipase Cγ1. A primary function of CD22 is thought to be in limiting antigen receptor signaling by modulating B cell activation threshold. CD22 has been shown to bind to CD45RO and CD75, although the natural ligands for this molecule remain controversial.\u003cbr\u003e\n100tests\u003cbr\u003e\nReactivity: Human\u003cbr\u003e\nReported Reactivity: Chimpanzee\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing True-Stain Monocyte Blocker™, 0.09% sodium azide and 0.2% (w\/v) BSA (origin USA) and a stabilizer.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: 200 µg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC- Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP\/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leukocyte Typing V:White Cell Differentiation Antigens. Oxford University Press. New York. Clark E. 1993. J. Immunol.. 150:4715. Shan D and O. Press. 1995. J. Immunol.. 154:4466.\u003cbr\u003e\nDisclaimer: GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include: Batch-to-batch consistency Material traceability Documented procedures Documented employee training Equipment maintenance and monitoring records Lot-specific certificates of analysis Quality audits per ISO 13485:2016 QA review of released products\u003cbr\u003e\nStructure: Ig superfamily, a type I glycosylated integral transmembrane protein, 120-130 kD\u003cbr\u003e\nDistribution: B cells\u003cbr\u003e\nFunction: Adhesion, B-monocyte and B-T interactions\u003cbr\u003e\nLigand\/Receptor: CD45RO, CD75\u003cbr\u003e\nCell Type: B cells\u003cbr\u003e\nBiology Area: Cell Adhesion, Cell Biology, Immunology\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules, Siglec Molecules\u003cbr\u003e\nAntigen References: 1. Clark E. 1993. J. Immunol. 150:4715. 2. Shan D, et al. 1995. J. Immunol. 154:4466.\u003cbr\u003e\nGene ID: 933\u003cbr\u003e\nUniProt: View information about CD22 on UniProt.org\u003cbr\u003e\nClone: HIB22\u003cbr\u003e\nWorkshop: V CD22.14\u003cbr\u003e\nOther Names: BL-CAM, Siglec-2, Lyb8\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866524012713,"sku":"260390","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-260390","provider":"Iright","version":"1.0","type":"link"}