{"product_id":"biolegend-260398","title":"Biolegend, 260398, GMP FITC anti-human CD138 (Syndecan-1) Antibody, 100tests","description":"\u003cp\u003eCD138, a member of the syndecan protein family, is a type I integral membrane heparin sulfate proteoglycan also known as Syndecan-1. Syndecan-1 participates in cell proliferation, cell migration, and cell-matrix adhesion via interaction with collagen, fibronectin, and other soluble molecules (such as FGF-basic). It is expressed on normal and malignant human plasma cells, pre-B cells, epithelial cells, and endothelial cells, but not on mature circulating B-lymphocytes. It is also expressed on some non-hematopoietic cells, including embryonic mesenchymal cells, vascular smooth muscle cells, endothelial cells, and neural cells.\u003cbr\u003e\n100tests\u003cbr\u003e\nReactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: A mixture of U266 and XG-1 human myeloma cell lines.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w\/v) BSA (origin USA) and a stabilizer.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.\u003cbr\u003e\nConcentration: 200 µg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: The epitope recognized by MI15 is found within the ectodomain of the CD138 core protein. It has been reported that MI15 blocks the binding of clone B-B4 but not clone DL-101 by flow cytometric analysis. Clones DL-101 and MI15 exhibit differential staining patterns on in vitro generated plasma cells and some CD138+ cell lines.4 Additional reported applications for the relevant formats include: immunofluorescent staining1, Western blotting2, immunohistochemical staining of formalin-fixed paraffin-embedded frozen tissue sections3, and spatial biology (IBEX)5,6.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Costes V, et al. 1999. Hum. Pathol. 30:1405. (IF) Gattei V, et al. 1999. Br. J. Haematol. 104:152. (WB) Bologna-Molina R, et al. 2008. Oral Oncol. 44:805. (IHC) Itoua MR, et al. 2014. Biomed. Res. Int. 2014:536482. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nDisclaimer: GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include: Batch-to-batch consistency Material traceability Documented procedures Documented employee training Equipment maintenance and monitoring records Lot-specific certificates of analysis Quality audits per ISO 13485:2016 QA review of released products\u003cbr\u003e\nStructure: 100-200 kD type I integral transmembrane glycoprotein\u003cbr\u003e\nDistribution: Plasma cells, pre-B cells, epithelial cells, endothelial cells\u003cbr\u003e\nFunction: Adhesion, controls cell morphology, regulates cell growth\u003cbr\u003e\nLigand\/Receptor: FGFb, collagen, fibronectin\u003cbr\u003e\nCell Type: B cells, Endothelial cells, Epithelial cells, Plasma cells\u003cbr\u003e\nBiology Area: Cell Adhesion, Cell Biology, Cell Motility\/Cytoskeleton\/Structure, Immunology, Neuroscience, Synaptic Biology\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Sanderson RD, et al. 1992. Cell. Regul. 1:27. 2. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules: The CD Markers. Wiley-Liss A John Wiley \u0026amp; Sons Inc, Publication.\u003cbr\u003e\nGene ID: 6382\u003cbr\u003e\nUniProt: View information about CD138 on UniProt.org\u003cbr\u003e\nClone: MI15\u003cbr\u003e\nWorkshop: HCDM listed\u003cbr\u003e\nOther Names: B-B4\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866526240937,"sku":"260398","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-260398","provider":"Iright","version":"1.0","type":"link"}