{"product_id":"biolegend-260400","title":"Biolegend, 260400, GMP PE anti-human IL-4 Antibody, 100tests","description":"\u003cp\u003eIL-4 is a pleiotropic cytokine that is produced by activated T cells, mast cells, and basophils. IL-4 elicits many different biological responses but has two dominant functions. The first is regulating differentiation of naïve CD4 + T cell to the Th2 type. Th2 cells produce IL-4, IL-5, IL-10, and IL-13, which tend to favor a humoral immune response while suppressing a cell-mediated immune response controlled by Th1 cells. The second is regulating IgE and IgG1 production by B cells.\u003cbr\u003e\n100tests\u003cbr\u003e\nReactivity: Human\u003cbr\u003e\nReported Reactivity: Pig, Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: CHO-expressed, recombinant human IL-4\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w\/v) BSA (origin USA) and a stabilizer.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 25 µg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: ELISA Detection1,3 or ELISPOT Detection4,5: The biotinylated MP4-25D2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified 8D4-8 antibody (Cat. No. 500702\/500707) as the capture antibody. Flow Cytometry6,9: The fluorochrome-labeled MP4-25D2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-4 -producing cells within mixed cell populations. Neutralization1-3: The LEAF™ purified antibody (Endotoxin \u0026lt;0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-4 bioactivity (Cat. No. 500815). The MP4-25D2 antibody can neutralize the bioactivity of natural or recombinant IL-4.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Chretien I, et al. 1989. J. Immunol. Methods 117:67. (ELISA Detection, Neut) Ramanathan L, et al. 1993. Biochem. 32:3549. (Neut) Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA Detection, Neut) Mahanty S, et al. 1992. J. Immunol. 148:3567. (ELISPOT Detection) Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.19. (ELISPOT Detection) Prussin C, et al. 1995. J. Immunol. Methods 188:117. (ICFC) Raqib R, et al. 1995. Infect. Immun. 63:289. Andersson J, et al. 1994. Immunology 83:16. Iwamoto S, et al. 2007. J. Immunol. 179:1449. (ICFC) PubMed Kubota M, et al. 1997. J. Immunol. 158:5321. Dzhagalov I, et al. 2007. J. Immunol. 178:2113. PubMed Kroneke MA, et al. 2012. J. Immunol. 188:3734. PubMed\u003cbr\u003e\nDisclaimer: GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include: Batch-to-batch consistency Material traceability Documented procedures Documented employee training Equipment maintenance and monitoring records Lot-specific certificates of analysis Quality audits per ISO 13485:2016 QA review of released products\u003cbr\u003e\nStructure: Cytokine; 15-19 kD (Mammalian)\u003cbr\u003e\nBioactivity: Differentiation of naïve CD4+ T cells to the TH2 type, proliferation\/differentiation of activated B cells, expression of class II MHC antigens, and of low affinity IgE receptors in resting B cells\u003cbr\u003e\nCell Sources: Mast cells, T cells, bone marrow stromal cells\u003cbr\u003e\nCell Targets: B cells, T cells, monocytes, endothelial cells, fibroblasts\u003cbr\u003e\nReceptors: Heterodimer IL-4Rα (CD124); γ-subunit (CD132) in common with IL-2R, IL-7R, IL-13R, IL-15R\u003cbr\u003e\nCell Type: Tregs\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Boulay J, et al. 1992. Curr. Opin. Immunol. 4:294. 3. Dullens H, et al. 1991. In vivo 5:567. 4. Paul W. 1991. Blood 77:1859.\u003cbr\u003e\nRegulation: Upregulated by IL-2, platelet activating factor; downregulated by TGF-β\u003cbr\u003e\nGene ID: 3565\u003cbr\u003e\nUniProt: View information about IL-4 on UniProt.org\u003cbr\u003e\nClone: MP4-25D2\u003cbr\u003e\nOther Names: Interleukin-4, Ia inducing factor (IaIF), B-cell stimulating factor-1 (BSF-1), Hodgkin's cell growth factor (HCGF), Mast cell growth factor-2 (MCGF-2), Macrophage fusion factor (MFF), T cell growth factor-2 (TCGF-2)\u003cbr\u003e\nIsotype: Rat IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866526404777,"sku":"260400","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-260400","provider":"Iright","version":"1.0","type":"link"}