{"product_id":"biolegend-280302","title":"Biolegend, 280302, Purified Streptavidin, 250μg","description":"\u003cp\u003eStreptavidin is a 52 kD tetrameric protein obtained from Streptomyces avidinii . It binds to biotin with a very high affinity and is one of the strongest interactions in nature with a dissociation constant of 10 -14 mol\/L. It is used in a wide range of applications including ELISA, flow cytometry, molecular biology, and bionanotechnology.\u003cbr\u003e\n250μg\u003cbr\u003e\nFormulation: Phosphate-buffered solution containing 0.09% sodium azide.\u003cbr\u003e\nConcentration: 1.0 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: Store between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested ELISA Capture, ELISA Detection, ICC, IHC-F, IHC-P, IP, WB - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: To coat one 96-well plate dilute 24 µL of Purified Streptavidin into 11.976ml of 1X Coating Buffer (Cat. No. 421701, Coating Buffer 5X concentrate). Final concentration in the coated plate is 2 µg\/mL. This recommendation is to perform the protocol for HLA class I ELISA to evaluate Flex-T™ peptide exchange. For other applications the reagent needs to be optimized.\u003cbr\u003e\nProduct Citations: Wu C, et al. 2022. ACS Nano. 16:1025. PubMed Khiew SH, et al. 2020. J Clin Invest. 130:3453. PubMed Wu C, et al. 2020. J Am Chem Soc. 142:12314. PubMed\u003cbr\u003e\nStructure: 52 kD tetrameric protein.\u003cbr\u003e\nDistribution: Streptavidin binds to biotin with high affinity.\u003cbr\u003e\nLigand\/Receptor: Biotin.\u003cbr\u003e\nAntigen References: 1. Dundas CM, et al. 2013. Appl. Microbiol. Biotechnol. 97:9343. 2. Zhao X, et al. 2013. J. Anal. Methods Chem. 2013:581093. 3. Kaplan DL, et al. 1999. Biomol. Eng. 16:135. 4. Wilbur DS, et al. 1999. Biomol. Eng. 16:113. 5. Sano T, et al. 1998. J. Chromatogr. B. Biomed. Sci. Appl. 715:85.\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Streptavidin-PURE, SAV-PU, SAV\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866502058153,"sku":"280302","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-280302","provider":"Iright","version":"1.0","type":"link"}