{"product_id":"biolegend-285032","title":"Biolegend, 285032, Spark Red™ 718 anti-mouse CD80 (Flexi-Fluor™) Antibody, 50μg","description":"\u003cp\u003eCD80 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily and is also known as B7-1, B7, and Ly-53. CD80 is constitutively expressed on dendritic cells and monocytes\/macrophages, and inducibly expressed on activated B and T cells. The ligation of CD28 on T cells with CD80 and CD86 (B7-2) on antigen presenting cells (such as dendritic cells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD80 appears to be expressed later in the immune response than CD86. CD80 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nReported Reactivity: Dog\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Armenian Hamster\u003cbr\u003e\nImmunogen: CHO cell line transfected with mouse B7 (CD80)\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Spark Red™ 718 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC\u003cbr\u003e\nRecommended Usage: Flexi-Fluors™ are provided at a standard 0.2 mg\/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg\/mL.For example, make five 1:1 serial dilutions of the 0.2 mg\/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells\/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration. * Spark Red™ 718 has a maximum excitation of 697 nm and a maximum emission of 711 nm.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation2, in vitro and in vivo blocking of CTLA-4 Ig to CD80 by blocking costimulation of T cells by activated B cells2-4, and immunohistochemical staining of acetone-fixed frozen sections1,4. The Ultra-LEAF™ purified antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 104747-104752).\u003cbr\u003e\nAdditional Product Notes: For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Harlan DM, et al. 1994. P. Natl. Acad. Sci. USA 91:3137. (IHC) Razi-Wolf Z, et al. 1992. P. Natl. Acad. Sci. USA 89:4210. (Block, IP) Hathcock KS, et al. 1994. J. Exp. Med. 180:631. (Block) Herold KC, et al. 1997. J. Immunol. 158:984. (Block, IHC) Ma XT, et al. 2006. Cancer Res. 66:1169. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC) Lawson BR, et al. 2007. J. Immunol. 178:5366. Turnquist HR, et al. 2007. J. Immunol. 178:7018. Misra RS, et al. 2010. J. Exp Med. 207:1775. PubMed del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed Philipsen L, et al. 2013. Mol Cell Proteomics. 12:2551. PubMed\u003cbr\u003e\nRRID: AB_3106297 (BioLegend Cat. No. 285032)\u003cbr\u003e\nStructure: Ig superfamily, 60 kD\u003cbr\u003e\nDistribution: Macrophages, activated B cells and T cells, dendritic cells\u003cbr\u003e\nFunction: T cell costimulation\u003cbr\u003e\nLigand\/Receptor: CD28 (stimulatory), CD152(CTLA4) (inhibitory)\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Macrophages, T cells, Tregs\u003cbr\u003e\nBiology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: CD Molecules, Immune Checkpoint Receptors\u003cbr\u003e\nAntigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Linsley PS, et al. 1991. J. Exp. Med. 174:561. 3. Salomon B, et al. 2001. Annu. Rev. Immunol. 19:225.\u003cbr\u003e\nGene ID: 12519\u003cbr\u003e\nUniProt: View information about CD80 on UniProt.org\u003cbr\u003e\nClone: 16-10A1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: B7-1, B7, Ly-53\u003cbr\u003e\nIsotype: Armenian Hamster IgG\u003cbr\u003e\nQ: What are Flexi-Fluors?\u003cbr\u003e\nA: Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.\u003cbr\u003e\nQ: How quickly will I receive my order?\u003cbr\u003e\nA: We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.\u003cbr\u003e\nQ: How are Flexi-Fluors different from regular catalog products?\u003cbr\u003e\nA: Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.\u003cbr\u003e\nQ: How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?\u003cbr\u003e\nA: Flexi-Fluors are provided at a standard 0.2 mg\/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg\/mL. \u003c\/p\u003e\n\n\u003cp\u003e \u003c\/p\u003e\n\n\u003cp\u003eFor example, make five 1:1 serial dilutions of your 0.2 mg\/mL antibody.  Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells\/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.\u003cbr\u003e\nQ: I can’t find the antibody-dye combination that I need. When will it be available?\u003cbr\u003e\nA: We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.\u003cbr\u003e\nQ: I need help to validate the performance of my Flexi-Fluor. Who should I contact?\u003cbr\u003e\nA: Please get in touch with Technical Service for assistance.\u003cbr\u003e\nQ: Can I order more than 50 μg of a Flexi-Fluor?\u003cbr\u003e\nA: Yes, you can order multiple vials of the same Flexi-Fluor products.  We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.\u003cbr\u003e\nQ: What is the expiration date of my Flexi-Fluor?\u003cbr\u003e\nA: Expiration dates can be found on the vial label or by using our CoA lookup tool.\u003cbr\u003e\nQ: What if my requested antibody-dye combination doesn't perform as expected?\u003cbr\u003e\nA: Flexi-Fluor™ products have been carefully selected by our scientists for their excellent conjugation properties and broad compatibility. In the rare case that an antibody-dye combination does not perform as expected due to the unique characteristics of a specific pairing, please contact our Technical Service team. We will work with you to identify alternative options to meet your panel requirements.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860815794345,"sku":"285032","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-285032","provider":"Iright","version":"1.0","type":"link"}