{"product_id":"biolegend-285129","title":"Biolegend, 285129, Spark Blue™ 574 anti-human CD181 (CXCR1) (Flexi-Fluor™) Antibody, 50μg","description":"\u003cp\u003eCXCR1 is a 67-70 kD seven-transmembrane protein, also known as IL-8 receptor A (IL-8RA), CD181, and CDw128a. It is a CXC chemokine receptor that belongs to the G protein-coupled receptor (CPCR) family. CXCR1 is expressed as homodimer or heterodimer with CXCR2 and found on granulocytes, NK cells, subset of T lymphocytes, mast cells, monocytes, endothelial cells, megakarocytes, and oligodendrocytes. The expression level of CXCR1 on monocytes is lower than that of CXCR2. CXCR1 mediates neutrophil activation and chemotaxis, megakaryocytic proliferation, and angiogenesis via binding its ligands including IL-8(CXCL8), NAP-2(CXCL7), GCP-2(CXCL6), and GRO-α(CXCL1).\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human CXCR1 transfected L1.2 cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 574 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC\u003cbr\u003e\nRecommended Usage: Flexi-Fluors™ are provided at a standard 0.2 mg\/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg\/mL.For example, make five 1:1 serial dilutions of the 0.2 mg\/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells\/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration. * Spark Blue™ 574 has a maximum excitation of 506 nm and a maximum emission of 574 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: The 8F1\/CXCR1 antibody is useful for immunofluorescent staining and flow cytometric analysis of CXCR1 expression.\u003cbr\u003e\nAdditional Product Notes: For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.\u003cbr\u003e\nRRID: AB_3662469 (BioLegend Cat. No. 285129)\u003cbr\u003e\nStructure: Seven transmembrane spanning, G protein-coupled receptor (CPCR), 67-70 kD, homodier or teterodimer with CXCR2\u003cbr\u003e\nDistribution: Granulocytes, NK cells, subset of T cells, monocytes, endothelial cells, oligodendrocytes\u003cbr\u003e\nFunction: neutrophil activation, chemotaxis, angiogenesis\u003cbr\u003e\nLigand\/Receptor: IL-8(CXCL8), NAP-2(CXCL7), GCP-2(CXCL6), GRO-a(CXCL1)\u003cbr\u003e\nCell Type: Endothelial cells, Granulocytes, Monocytes, Neutrophils, NK cells, Oligodendrocytes, T cells\u003cbr\u003e\nBiology Area: Angiogenesis, Cell Biology, Immunology, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors, GPCR\u003cbr\u003e\nAntigen References: 1. Chuntharapai A, et al. 1994. J. Immunol. 153:5682. 2. Wilson S, et al. 2005. J. Biol. Chem. 280:28663. 3. Emadi S, et al. 2005. Blood 105:464. 4. Omari KM, et al. 2005. Brain 128:1003. 5. Juremalm M, G. Nilsson. 2005. Chem. Immunol. Allergy. 87:130. 6. Wolf M, et al. 1998. Eur. J. Immunol. 28:164\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nUniProt: View information about CD181 on UniProt.org\u003cbr\u003e\nClone: 8F1\/CXCR1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: CD181, CXCR1, CDw128, CD128a, IL-8RA, Type I IL-8 receptor\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003cbr\u003e\nQ: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?\u003cbr\u003e\nA: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.\u003cbr\u003e\nQ: What are Flexi-Fluors?\u003cbr\u003e\nA: Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.\u003cbr\u003e\nQ: How quickly will I receive my order?\u003cbr\u003e\nA: We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.\u003cbr\u003e\nQ: How are Flexi-Fluors different from regular catalog products?\u003cbr\u003e\nA: Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.\u003cbr\u003e\nQ: How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?\u003cbr\u003e\nA: Flexi-Fluors are provided at a standard 0.2 mg\/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg\/mL. \u003c\/p\u003e\n\n\u003cp\u003e \u003c\/p\u003e\n\n\u003cp\u003eFor example, make five 1:1 serial dilutions of your 0.2 mg\/mL antibody.  Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells\/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.\u003cbr\u003e\nQ: I can’t find the antibody-dye combination that I need. When will it be available?\u003cbr\u003e\nA: We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.\u003cbr\u003e\nQ: I need help to validate the performance of my Flexi-Fluor. Who should I contact?\u003cbr\u003e\nA: Please get in touch with Technical Service for assistance.\u003cbr\u003e\nQ: Can I order more than 50 μg of a Flexi-Fluor?\u003cbr\u003e\nA: Yes, you can order multiple vials of the same Flexi-Fluor products.  We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.\u003cbr\u003e\nQ: What is the expiration date of my Flexi-Fluor?\u003cbr\u003e\nA: Expiration dates can be found on the vial label or by using our CoA lookup tool.\u003cbr\u003e\nQ: What if my requested antibody-dye combination doesn't perform as expected?\u003cbr\u003e\nA: Flexi-Fluor™ products have been carefully selected by our scientists for their excellent conjugation properties and broad compatibility. In the rare case that an antibody-dye combination does not perform as expected due to the unique characteristics of a specific pairing, please contact our Technical Service team. We will work with you to identify alternative options to meet your panel requirements.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862494662825,"sku":"285129","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-285129","provider":"Iright","version":"1.0","type":"link"}