{"product_id":"biolegend-285140","title":"Biolegend, 285140, Spark Blue™ 574 anti-mouse CD185 (CXCR5) (Flexi-Fluor™) Antibody, 50μg","description":"\u003cp\u003eCD185 is also known as CXCR5. It is the receptor for chemokine CXCL13\/BLC, which is chemotactic for B cells.  CXCR5 is expressed on B cells and a subset of T cells in the spleen, neuronal tissue, lymph nodes, and bone marrow.  It is important for migration of B cells into the B cell follicles of the spleen and Peyer’s patches.  Follicular helper T cells (Tfh) also express CXCR5 and the ability of these cells to migrate to the lymph node is modulated by the balanced expression of CCR7 and CXCR5.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: mCXCR5-transfected cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 574 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC\u003cbr\u003e\nRecommended Usage: Flexi-Fluors™ are provided at a standard 0.2 mg\/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg\/mL.For example, make five 1:1 serial dilutions of the 0.2 mg\/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells\/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration. * Spark Blue™ 574 has a maximum excitation of 506 nm and a maximum emission of 574 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Clone L138D7 staining works optimally at room temperature or 4°C. Unlike other chemokine receptor antibodies, avoid using L138D7 at 37°C.\u003cbr\u003e\nAdditional Product Notes: For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.\u003cbr\u003e\nRRID: AB_3662480 (BioLegend Cat. No. 285140)\u003cbr\u003e\nStructure: 42 kD, seven transmembrane protein\u003cbr\u003e\nDistribution: B cells and a subset of T cells in mouse spleen, Tfh cells, also expressed in lymph node, bone marrow, and brain\u003cbr\u003e\nFunction: B cell migration into follicles, possible regulatory role in B cell differentiation and Burkitt's lymphoma\u003cbr\u003e\nLigand\/Receptor: BLC\/CXCL13\u003cbr\u003e\nCell Type: B cells, T cells, Tfh\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: CD Molecules, Cytokine\/Chemokine Receptors, GPCR\u003cbr\u003e\nAntigen References: 1. Kaiser E, et al. 1993. Eur. J. Immunol. 23:2532. 2. Forster R, et al. 1994. Cell. Mol. Biol. 40:381. 3. Forster R, et al. 1994. Blood 84:830. 4. Forster R, et al. 1996. Cell 87:1037. 5. Wolf I, et al. 1998. J. Biol. Chem. 273:28831.\u003cbr\u003e\nGene ID: 12145\u003cbr\u003e\nUniProt: View information about CD185 on UniProt.org\u003cbr\u003e\nClone: L138D7\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: BLR1, GPCR6, MDR-15\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003cbr\u003e\nQ: What are Flexi-Fluors?\u003cbr\u003e\nA: Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.\u003cbr\u003e\nQ: How quickly will I receive my order?\u003cbr\u003e\nA: We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.\u003cbr\u003e\nQ: How are Flexi-Fluors different from regular catalog products?\u003cbr\u003e\nA: Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.\u003cbr\u003e\nQ: How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?\u003cbr\u003e\nA: Flexi-Fluors are provided at a standard 0.2 mg\/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg\/mL. \u003c\/p\u003e\n\n\u003cp\u003e \u003c\/p\u003e\n\n\u003cp\u003eFor example, make five 1:1 serial dilutions of your 0.2 mg\/mL antibody.  Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells\/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.\u003cbr\u003e\nQ: I can’t find the antibody-dye combination that I need. When will it be available?\u003cbr\u003e\nA: We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.\u003cbr\u003e\nQ: I need help to validate the performance of my Flexi-Fluor. Who should I contact?\u003cbr\u003e\nA: Please get in touch with Technical Service for assistance.\u003cbr\u003e\nQ: Can I order more than 50 μg of a Flexi-Fluor?\u003cbr\u003e\nA: Yes, you can order multiple vials of the same Flexi-Fluor products.  We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.\u003cbr\u003e\nQ: What is the expiration date of my Flexi-Fluor?\u003cbr\u003e\nA: Expiration dates can be found on the vial label or by using our CoA lookup tool.\u003cbr\u003e\nQ: What if my requested antibody-dye combination doesn't perform as expected?\u003cbr\u003e\nA: Flexi-Fluor™ products have been carefully selected by our scientists for their excellent conjugation properties and broad compatibility. In the rare case that an antibody-dye combination does not perform as expected due to the unique characteristics of a specific pairing, please contact our Technical Service team. We will work with you to identify alternative options to meet your panel requirements.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866118279337,"sku":"285140","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-285140","provider":"Iright","version":"1.0","type":"link"}