{"product_id":"biolegend-300423","title":"Biolegend, 300423, Alexa Fluor® 700 anti-human CD3 Antibody, 25μg","description":"\u003cp\u003eCD3ε is a 20 kD chain of the CD3\/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α\/β or γ\/δ) heterodimer. It is found on all mature T cells, NKT cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: Chimpanzee\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The CD3 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm \/ 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, Western blotting9, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin \u0026lt; 0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin \u0026lt; 0.01 EU\/µg).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP) Graves J, et al. 1991. J. Immunol. 146:2102. (Activ) Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ) Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC) Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ) Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC) Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC) Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed Van Dongen JJM, et al. 1988. Blood 71:603. (WB) Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC) Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed Li J, et al. 2015. Cancer Res. 75:508. PubMed Stoeckius M, et al. 2017. Nat. Methods. 14:865-868. (PG) Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Laprevotte E, et al. 2013. Leuk Res. 37:440. PubMed Rappazzo CG, et al. 2020. bioRxiv. . PubMed Iwahori K, et al. 2019. Sci Rep. 2.205555556. PubMed Tan GMY, et al. 2022. Cancers (Basel). 14:. PubMed Xiong H, et al. 2023. EBioMedicine. 90:104507. PubMed Menevse AN, et al. 2023. Acta Neuropathol Commun. 11:75. PubMed Kim JH, et al. 2020. Sci Rep. 10:1835. PubMed Chen Y, et al. 2022. Sci Immunol. 7:eabp8328. PubMed Labuz DR, et al. 2023. Elife. 12: . PubMed Chiu YL, et al. 2018. Immun Ageing. 15:27. PubMed Ivan Jelcic et al. 2018. Cell. 175(1):85-100 . PubMed Serra–Peinado C, et al. 2019. Nat Commun. 10:3705. PubMed Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed Walsh RE, et al. 2020. MAbs. 12:1764829. PubMed Mashiko S, et al. 2015. J Allergy Clin Immunol. 136: 351-359. PubMed Keustermans G,et al. 2017. PLoS One. 10.1371\/journal.pone.0187068. PubMed Di Blasi D, et al. 2019. Cell Mol Gastroenterol Hepatol. 0.510416667. PubMed Jacobs ME, et al. 2021. Immunother Adv. 1:ltaa004. PubMed Sundström P, et al. 2015. J Immunol. 195: 3472 - 3481. PubMed Zhou J, et al. 2015. J Immunol. 194:4688. PubMed Judge CJ, et al. 2017. J Leukoc Biol. 102:171. PubMed Na I, et al. 2013. Haematologica. 98:23. PubMed Hansen IS, et al. 2018. Nat Commun. 9:863. PubMed Chandele A, et al. 2016. J Virol. 90(24):11259-11278. PubMed Kiniry B, et al. 2016. Mucosal Immunol. 10.1038\/mi.2016.100. PubMed Roberta Zappasodi et al. 2018. Cancer cell. 33(6):1017-1032 . PubMed Rajamanickam V, et al. 2021. Cancer Immunol Res. 9:602. PubMed Kacherovsky N, et al. 2019. Nat Biomed Eng. 0.66875. PubMed Lutter L, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:1567. PubMed Studniberg SI, et al. 2022. Mol Syst Biol. 18:e10824. PubMed OConnor RA, et al. 2021. OncoImmunology. 10(1):1940675. PubMed Cheng Y, et al. 2021. Immunity. 54(8):1825-1840.e7. PubMed Khaitan A, et al. 2016. PLoS One. 11: 0161786. PubMed Hazenberg MD, et al. 2019. Blood Adv. 2.659722222. PubMed Bochem J, et al. 2019. PLoS One. 14:e0221301. PubMed Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed Leclerc M, et al. 2019. Nat Commun. 10:3345. PubMed Krishnan S, et al. 2021. Clin Exp Immunol. 203:458. PubMed Golebski K, et al. 2021. Immunity. 54(2):291-307.e7. PubMed Yoshida R, et al. 2022. Cancer Res. :. PubMed Gorman JA, et al. 2019. Front Immunol. 10:44. PubMed Khuzwayo S, et al. 2021. Front Immunol. 12:631410. PubMed Fang F, et al. 2021. Cell Rep. 37:109981. PubMed Verma K, et al. 2017. PLoS One.. 10.1371\/journal.pone.0183828. PubMed Berry MR et al. 2017. Cell. 170(5):860-874 . PubMed Janssen JJE, et al. 2022. Am J Physiol Endocrinol Metab. 322:E141. PubMed Chen Y, et al. 2020. Cell. 1496:183. PubMed Hood SP, et al. 2020. Elife. 9:00. PubMed Chen J, et al. 2017. Sci Rep. 10.1038\/s41598-017-11056-9. PubMed FitzPatrick MEB, et al. 2021. Cell Rep. 34:108661. PubMed Bal S, et al. 2016. Nat Immunol. 10.1038\/ni.3444. PubMed Rousset F, et al. 2018. Mol Ther Nucleic Acids. 14:351. PubMed Goc J, et al. 2021. Cell. 184:5015. PubMed Martin GE, et al. 2018. Front Immunol. 1.019444444. PubMed Harris LD, et al. 2020. Front Cell Infect Microbiol. 10:120. PubMed Corgnac S, et al. 2021. STAR Protocols. 2(1):100267. PubMed Tsai S, et al. 2018. Cell Metab. 28:922. PubMed\u003cbr\u003e\nRRID: AB_493740 (BioLegend Cat. No. 300423) AB_493741 (BioLegend Cat. No. 300424)\u003cbr\u003e\nStructure: Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α\/β or γ\/δ) forms CD3\/TCR complex, 20 kD\u003cbr\u003e\nDistribution: Mature T and NK T cells, thymocyte differentiation\u003cbr\u003e\nFunction: Antigen recognition, signal transduction, T cell activation\u003cbr\u003e\nLigand\/Receptor: Peptide antigen bound to MHC\u003cbr\u003e\nCell Type: NKT cells, T cells, Thymocytes, Tregs\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: CD Molecules, TCRs\u003cbr\u003e\nAntigen References: 1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego. 2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329. 3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.\u003cbr\u003e\nGene ID: 916\u003cbr\u003e\nUniProt: View information about CD3 on UniProt.org\u003cbr\u003e\nClone: UCHT1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: III 471\u003cbr\u003e\nOther Names: T3, CD3ε\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864074047657,"sku":"300423","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-300423","provider":"Iright","version":"1.0","type":"link"}