{"product_id":"biolegend-301422","title":"Biolegend, 301422, PE\/Dazzle™ 594 anti-human CD11b (activated) Antibody, 100tests","description":"\u003cp\u003eThe CBRM1\/5 antibody reacts with an activated form of human CD11b, a 165-170 kD type I transmembrane glycoprotein also known as α M integrin, Mac-1, CR3, and C3biR. CD11b non-covalently associates with integrin β 2 (CD18) and is expressed on granulocytes, monocytes\/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b\/CD18 is critical for the transendothelial migration of monocytes and neutrophils as well as adhesion, phagocytosis, and neutrophil activation. CD11b\/CD18 interacts with ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen and factor X.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE\/Dazzle™ 594 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * PE\/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: The CBRM1\/5 antibody recognizes a subset of CD11b molecules on neutrophils and monocytes activated with chemoattractants or phorbol-esters. This antibody does not recognize \"non-activated\" CD11b. The epitope recognized by CBRM1\/5 is contained in the I domain of the a chain. Clone CBRM1\/5 binds the I domain, close to the ligand binding site. It recognizes a conformational change of the integrin9. Additional reported applications (for the relevant formats) include: immunoprecipitation, and blocking cell adhesion to fibrinogen and ICAM-11. The CBRM1\/5 antibody binds specifically to the activated form of CD11b. The LEAF™ purified antibody (Endotoxin \u0026lt;0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301408).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Diamond M, et al. 1993. J. Cell Biol. 120:545. (Block) Bryn T, et al. 2006. J. Immunol. 176:7361. PubMed Sithu SD, et al. 2007. J. Biol. Chem. doi:10.1074\/jbc.M611273200. Barthel SR,et al.2006. Am J. Respir Cell Mol Biol.35:378. PubMed Pillay J,et al. 2010. J. Leukocyte Biol. 88:211. PubMed Wilson RP, et al. 2011. Infect Immun. 79:830. PubMed Koleva RI, et al. 2012. Blood. 119:4878. PubMed. Eleftherious D, et al. 2012. Neurology. 79:2089. PubMed Oxvig C, et al. 1999. Proc. Natl. Acad. Sci. USA 96:2215. Muller-Edenborn B, et al. 2014. Br J Anaesth. PubMed Cash JL, et al. 2013. EMBO Rep. 14:999. (FC) PubMed\u003cbr\u003e\nRRID: AB_2800733 (BioLegend Cat. No. 301421) AB_2800734 (BioLegend Cat. No. 301422)\u003cbr\u003e\nStructure: Integrin, type I transmembrane glycoprotein, associates with integrin β2 (CD18), 165-170 kD\u003cbr\u003e\nDistribution: Granulocytes, monocytes\/macrophages, dendritic cells, NK cells, T cell subset, B cell subset\u003cbr\u003e\nFunction: Adhesion, phagocytosis, chemotaxis, neutrophil activation\u003cbr\u003e\nLigand\/Receptor: ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, factor X\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, NK cells, T cells, Tregs\u003cbr\u003e\nBiology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Stewart M, et al. 1995. Curr. Opin. Cell Biol. 7:690. 2. Petty H, et al. 1996. Immunol. Today 17:209.\u003cbr\u003e\nGene ID: 3684\u003cbr\u003e\nUniProt: View information about CD11b on UniProt.org\u003cbr\u003e\nClone: CBRM1\/5\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Integrin αM chain, C3biR, CR3, Mac-1, Mo1, ITGAM\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862972911785,"sku":"301422","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-301422","provider":"Iright","version":"1.0","type":"link"}