{"product_id":"biolegend-302301","title":"Biolegend, 302301, Purified anti-human CD20 Antibody, 25μg","description":"\u003cp\u003eCD20 is a 33-37 kD, four transmembrane spanning protein, also known as B1 and Bp35. CD20 is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated on activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca 2+ conductive ion channels in the plasma membrane of B cells. The CD20 molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). It exists in a complex with MHC class I and II, CD53, CD81, and CD82.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human, Cynomolgus, Rhesus\u003cbr\u003e\nReported Reactivity: Baboon, Capuchin Monkey, Chimpanzee, Pigtailed Macaque, Squirrel Monkey\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human tonsillar B cells\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested CyTOF®, IHC-F - Verified IP - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: The epitope recognized by clone 2H7 has been mapped to the sequence YNCEPANPSEKNSPST which lies in the large extracellular loop of human CD20. Additional reported applications (for the relevant formats) include: immunoprecipitation4 and immunohistochemical staining of acetone-fixed frozen sections5.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. McMichael A, et al. Eds. 1987. Leucocyte Typing III Oxford University Press. New York. Polyak MJ, et al. 2002. Blood 99:3256. (IP) Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)\u003cbr\u003e\nProduct Citations: Tornack J, et al. 2017. PLoS One. 12:e0169119. PubMed McIlwain DR, et al. 2021. Cell Host Microbe. 29:1828. PubMed McCarthy EE, et al. 2022. Cell Rep. 39:110815. PubMed Taft J, et al. 2021. Cell. 184(17):4447-4463.e20. PubMed Zhong J, et al. 2018. Sci Adv. 4:eaas9864. PubMed Alcántara-Hernández M, et al. 2021. Nat Protoc. 16:4855. PubMed Ercolano G, et al. 2021. Nat Commun. 12:2538. PubMed Gruber CN, et al. 2020. Immunity. 53(3):672-684. PubMed Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed Gunawan M, et al. 2017. Sci Rep. . 10.1038\/s41598-017-16999-7. PubMed Kong R, et al. 2019. Cell. 178:567. PubMed Bruno TC, et al. 2017. Cancer Immunol Res. 0.831944444. PubMed Cottineau J, et al. 2017. J Clin Invest. 127:1991. PubMed Olin A, et al. 2018. Cell. 174:1277. PubMed Agrawal N, et al. 2018. Front Immunol. 2.053472222. PubMed Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed Ehinger E, et al. 2021. Cardiovasc Res. 117:1166. PubMed Henrick BM, et al. 2021. Cell. . PubMed Chng MHY, et al. 2020. Immunity. 51(6):1119-1135.e5.. PubMed Lee YS, et al. 2021. J Immunother Cancer. 9:. PubMed Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed Manes TL, et al. 2020. Free Radic Biol Med. 147:102. PubMed Saber MM, et al. 2022. Antibodies (Basel). 11:. PubMed Molina MS, et al. 2021. Front Immunol. 12:699128. PubMed Murayama M, et al. 2015. Nat Commun. 6: 8483. PubMed Chevrier S, et al. 2021. Cell Reports Medicine. 2(1):100166. PubMed Sander J et al. 2017. Immunity. 47(6):1051-1066 . PubMed Joas S, et al. 2020. Cell Rep. 30:2261. PubMed Ravell JC, et al. 2020. J Clin Invest. 130:507. PubMed Ainai A, et al. 2020. Microbiol Immunol. 64:313. PubMed Li Z, et al. 2020. J Clin Lab Anal. 34:e23155. PubMed\u003cbr\u003e\nRRID: AB_314249 (BioLegend Cat. No. 302301) AB_314250 (BioLegend Cat. No. 302302)\u003cbr\u003e\nStructure: Four transmembrane protein (TM4SF), heavily phosphorylated after activation, 33-37 kD\u003cbr\u003e\nDistribution: B cell, T cell subsets\u003cbr\u003e\nFunction: B cell activation\u003cbr\u003e\nLigand\/Receptor: Src family tyrosine kinases, MHC class I, II, CD53, CD81, CD82\u003cbr\u003e\nCell Type: B cells, T cells\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Hultin L, et al. 1993. Cytometry 14:196. 2. Tedder T, et al. 1994. Immunol. Today 15:450.\u003cbr\u003e\nGene ID: 931\u003cbr\u003e\nUniProt: View information about CD20 on UniProt.org\u003cbr\u003e\nClone: 2H7\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nWorkshop: IV B201\u003cbr\u003e\nOther Names: B1, Bp35\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003cbr\u003e\nQ: What is the difference between two anti human CD20 clones 2H7 and 1412?\u003cbr\u003e\nA: For clone 1412: This clone specifically recognizes cytoplasmic domain of CD20 and thus can only be used for intracellular flow cytometry. In this instance you will need to include the fixation and permeabilization steps. Please follow the intracellular flow cytometry staining protocol.\u003c\/p\u003e\n\n\u003cp\u003eFor clone 2H7: This clone is ok for regular surface staining for CD20 and there is no need for any fixation and permeabilization steps.\u003c\/p\u003e\n\n\u003cp\u003eOur technical protocols can be found here.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862329610409,"sku":"302301","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/es\/products\/biolegend-302301","provider":"Iright","version":"1.0","type":"link"}